Metallic nanoparticles such as silver (Ag NPs) and iron oxide (Fe₃O₄ NPs) nanoparticles are high production volume materials due to their applications in various consumer products, and in nanomedicine. However, their inherent toxicities to human cells remain a challenge. The present study was aimed at combining lipidomics data with common phenotypically-based toxicological assays to gain better understanding into cellular response to Ag NPs and Fe₃O₄ NPs exposure. HepG2 cells were exposed to different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 µg/ml) of the nanoparticles for 24 h, after which they were assayed for toxic effects using toxicological assays like cytotoxicity, mutagenicity, apoptosis and oxidative stress. The cell membrane phospholipid profile of the cells was also performed using shotgun tandem mass spectrometry. The results showed that nanoparticles exposure resulted in concentration-dependent cytotoxicity as well as reduced cytokinesis-block proliferation index (CBPI). Also, there was an increase in the production of ROS and superoxide anions in exposed cells compared to the negative control. The lipidomics data revealed that nanoparticles exposure caused a modulation of the phospholipidome of the cells. A total of 155 lipid species were identified, out of which the fold changes of 23 were significant. The high number of differentially changed phosphatidylcholine species could be an indication that inflammation is one of the major mechanisms of toxicity of the nanoparticles to the cells.

金属纳米颗粒，例如银（Ag NPs）和氧化铁（Fe ₃ O ₄ NPs）纳米颗粒，由于其在各种消费产品和纳米药物中的应用而成为高产量的材料。然而，它们对人类细胞的固有毒性仍然是一个挑战。本研究旨在将脂质组学数据与常见的基于表型的毒理学分析相结合，以更好地了解细胞对Ag NP和Fe ₃ O _4的反应NP暴露。将HepG2细胞暴露于不同浓度（3.125、6.25、12.5、25、50和100 µg / ml）的纳米颗粒中24小时，然后使用毒理学方法（如细胞毒性，诱变性，细胞凋亡和氧化应激）对它们的毒性作用进行分析。 。还使用shot弹枪串联质谱法进行细胞的细胞膜磷脂谱分析。结果表明，纳米颗粒暴露导致浓度依赖性细胞毒性以及减少的胞质阻滞增殖指数（CBPI）。而且，与阴性对照相比，裸露的细胞中ROS和超氧阴离子的产生增加。脂质组学数据显示，纳米颗粒暴露引起细胞磷脂组的调节。总共鉴定出155种脂质，其中23倍的变化很明显。大量差异变化的磷脂酰胆碱物种可能表明炎症是纳米颗粒对细胞毒性的主要机制之一。