Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, play important roles in symbioses between higher plants and mycorrhizal fungi, and stimulate germination of plant–parasitic Orobanche and Striga species. Chemical analysis is central to research on the biochemistry of SLs and their roles in developmental biology and plant physiology. Here we summarize key issues relating to the identification and quantification of SLs isolated from plant tissues and exudates. The advantages and drawbacks of different protocols used for strigolactone analysis are discussed, and guidelines for selecting a procedure that will minimize losses during isolation and purification prior to final analysis are proposed. Hyphenated techniques suitable for SL analysis such as GC–MS and LC–MS/MS are also discussed, and newer ambient techniques such as HR-DART-MS and DESI-MS are highlighted as tools with considerable potential in SL research. A key advantage of these methods is that they require only simply sample preparation.

中文翻译：

---

松果内酯研究中的分析方法。

Strigolactones（SLs）是重要的植物激素，它是通过类胡萝卜素的生物合成途径产生的，并且在各种植物物种中的浓度都非常低。它们调节根的发育，在高等植物与菌根真菌之间的共生中起重要作用，并刺激植物寄生的Orobanche和Striga物种的萌发。化学分析对于SL的生物化学及其在发育生物学和植物生理学中的作用研究至关重要。在这里，我们总结了与从植物组织和分泌物中分离的SL的鉴定和定量有关的关键问题。讨论了用于磺基内酯分析的不同规程的优缺点，并提出了在最小化最终分析之前选择分离和纯化过程中损失最小的方法的指南。还讨论了适用于SL分析的联用技术，例如GC-MS和LC-MS / MS，并着重介绍了较新的环境技术，例如HR-DART-MS和DESI-MS，它们在SL研究中具有巨大的潜力。这些方法的主要优点是它们仅需要简单的样品制备。