Cannabis sativa is a dioecious plant with a XY system. Only females produce cannabinoids in large amount. Efficient male removal is an important issue for the cannabis industry. We have recently identified the sex chromosomes of C. sativa, which opens opportunities for developing universal genetic markers for early sexing of C. sativa plants. Here we selected six Y-linked markers and designed PCR primers, which were tested on five hemp cultivars both dioecious and monoecious. We obtained promising results, which need to be extended using a larger number of individuals and a more diverse set of cultivars, including THC producing ones.

中文翻译：

---

大麻种子性育遗传标记的开发

大麻是具有XY系统的雌雄异株植物。只有雌性会大量生产大麻素。有效去除男性是大麻产业的重要问题。我们最近确定了苜蓿中华草的性染色体，这为开发为苜蓿中华草的早期性育的通用遗传标记打开了机会。在这里，我们选择了六个Y连锁标记，并设计了PCR引物，分别在五个雌雄异株的大麻品种上进行了测试。我们获得了令人鼓舞的结果，需要使用更多的个体和更多样化的品种（包括THC生产品种）进行扩展。