Parallel information processing is a salient feature of complex nervous systems. For example, the medial and lateral hippocampal networks (MHN and LHN) preferentially process spatial- and object-related information, respectively. However, the mechanisms underlying parallel network assembly during development remain largely unknown. Here, we show that complementary expression of cell-surface molecules Teneurin-3 (Ten3) and Latrophilin-2 (Lphn2) in the MHN and LHN, respectively, guides the precise assembly of both the MHN and LHN. Viral-genetic perturbations in vivo demonstrate that Ten3+ axons are repelled by target-derived Lphn2, revealing that Lphn2/Ten3-mediated repulsion and Ten3/Ten3-mediated attraction cooperate to control precise target selection of MHN axons. In the LHN, Lphn2+ axons are confined to Lphn2+ targets via repulsion from Ten3+ targets. Our findings demonstrate that assembly of parallel hippocampal networks follows a 'Ten3→Ten3, Lphn2→Lphn2' rule instructed by reciprocal repulsions.

中文翻译：

---

相互排斥指示平行海马网络的精确组装

并行信息处理是复杂神经系统的显着特征。例如，内侧和外侧海马网络（MHN和LHN）分别优先处理与空间和对象有关的信息。但是，在开发过程中并行网络组装的基础机制仍然未知。在这里，我们表明细胞表面分子Teneurin-3（Ten3）和Latrophilin-2（Lphn2）在MHN和LHN中的互补表达分别指导MHN和LHN的精确组装。体内的病毒遗传扰动表明，Ten3 +轴突被靶标衍生的Lphn2排斥，这表明Lphn2 / Ten3介导的排斥力和Ten3 / Ten3介导的吸引力共同控制了MHN轴突的精确靶标选择。在LHN中，Lphn2 +轴突通过从Ten3 +靶标排斥而局限于Lphn2 +靶标。我们的发现表明，平行海马网络的组装遵循相互排斥指示的“ Ten3→Ten3，Lphn2→Lphn2”规则。