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Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms
bioRxiv - Genomics Pub Date : 2020-05-28 , DOI: 10.1101/2020.05.25.114793
R. Rebecca Love , Marco Pombi , Moussa W. Guelbeogo , Nathan R. Campbell , Melissa T. Stephens , Roch K. Dabire , Carlo Costantini , Alessandra della Torre , Nora J. Besansky

Chromosomal inversion polymorphisms have special importance in the Anopheles gambiae complex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. The study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. To overcome this barrier, we ascertained tag single nucleotide polymorphisms (SNPs) that are highly correlated with inversion status (inverted or standard orientation). We compared the performance of the tag SNPs using two alternative high throughput molecular genotyping approaches versus traditional cytogenetic karyotyping of the same 960 individual An. gambiae and An. coluzzii mosquitoes sampled from Burkina Faso, West Africa. We show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. Given the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag SNPs has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation of An. gambiae and An. coluzzii to environmental heterogeneities.

中文翻译:

使用高通量阵列和测序平台对冈比亚按蚊复合体的反基因分型

染色体倒位多态性在疟疾媒介蚊的冈比亚按蚊群中具有特别重要的地位,这是由于它们在局部适应和范围扩大中的作用。对自然种群倒位的研究依赖于专业细胞遗传学家对多态染色体的分析,这一过程受到训练有素的专家稀少,通量低和采样要求严格的限制。为了克服这一障碍,我们确定了与单核苷酸多态性(SNPs)高度相关的反转状态(反转或标准方向)。我们比较了使用两种替代的高通量分子基因分型方法与相同960个人An的传统细胞遗传核型方法的标签SNP的性能。冈比亚和安。从西非布基纳法索取样的蚊子。我们表明,两种分子方法都能产生可比的结果,并且在基因分型准确度方面,任何一种方法都比细胞遗传学表现更好或更好。鉴于分子基因分型方法能够在不限制蚊子性别或发育阶段的情况下以大规模和低成本进行的研究,通过标签SNPs进行分子基因分型有可能振兴关于染色体倒置在行为和持续适应中的作用的研究。一个。冈比亚和安。导致环境异质性。鉴于分子基因分型方法能够在不限制蚊子性别或发育阶段的情况下以大规模和低成本进行的研究,通过标签SNPs进行分子基因分型有可能振兴关于染色体倒置在行为和持续适应中的作用的研究。一个。冈比亚和安。导致环境异质性。鉴于分子基因分型方法能够在不限制蚊子性别或发育阶段的情况下以大规模和低成本进行的研究,通过标签SNPs进行分子基因分型有可能振兴关于染色体倒置在行为和持续适应中的作用的研究。一个。冈比亚和安。导致环境异质性。
更新日期:2020-05-28
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