Soybean root rot is a typical soil-borne disease that severely affects the yield of soybean, and F. mosseae, the dominant strain of AMF in continuous cropping of soybean. The aim of this study was to providing an experimental basis for the study of the molecular mechanism underlying the alleviation of the obstacles associated with the continuous cropping of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean infected with F. oxysporum. The results showed that the incidence of soybean root rot was significantly reduced after inoculation with F. mosseae. The significantly upregulated genes encoded the ABC transporter, ATP-binding/permease protein and the ABC transporter, ATP-binding protein. The significantly downregulated genes encoded chitin-binding domain proteins; key enzymes involved in metabolic pathways such as glycolysis, including class II fructose-bisphosphate aldolase and NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, glycoside hydrolase family 61 protein, which hydrolyse cellulose and hemicellulose; actin and other major components of the cytoskeleton. The DEGs were enriched in antigen processing and presentation, carbon fixation in photosynthetic organisms, glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could promote the growth and development of soybean and improve disease resistance. This study provides an experimental basis for further research on the molecular mechanism underlying the alleviation of challenges associated with the continuous cropping of soybean by AMF.

中文翻译：

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转录组学分析显示，Funneliformis mosseae对尖孢镰刀菌差异表达基因的影响

大豆根腐病是一种典型的土壤传播疾病，会严重影响大豆和F. mosseae的产量，而mosseae是连续种植AMF的主要菌株。这项研究的目的是为研究AMF缓解与大豆连续种植相关的障碍的分子机制提供实验基础。在这项研究中，将mosseae接种在种植了被oxysporum感染的大豆的土壤中。结果表明，接种mosseae后，大豆根腐病的发生率明显降低。显着上调的基因编码ABC转运蛋白ATP结合/渗透酶蛋白和ABC转运蛋白ATP结合蛋白。显着下调的基因编码了几丁质结合域蛋白。涉及糖酵解等代谢途径的关键酶，包括II类果糖-双磷酸醛缩酶和NAD依赖性甘油醛-3-磷酸脱氢酶，糖苷水解酶家族61蛋白，可水解纤维素和半纤维素；肌动蛋白和细胞骨架的其他主要成分。DEG富含抗原加工和呈递，光合生物中的碳固定，糖酵解/糖异生，MAPK信号通路，内质网中的蛋白质加工和RNA降解。mosseae F. mosseae的接种可以促进大豆的生长发育并提高抗病性。这项研究为进一步研究缓解AMF连续种植大豆带来的挑战的分子机制提供了实验基础。包括II类果糖二磷酸醛缩酶和NAD依赖性甘油醛-3-磷酸脱氢酶，糖苷水解酶家族61蛋白，可水解纤维素和半纤维素；肌动蛋白和细胞骨架的其他主要成分。DEG富含抗原加工和呈递，光合生物中的碳固定，糖酵解/糖异生，MAPK信号通路，内质网中的蛋白质加工和RNA降解。F. mosseae的接种可以促进大豆的生长发育并提高抗病性。这项研究为进一步研究缓解AMF连续种植大豆带来的挑战的分子机制提供了实验基础。包括II类果糖二磷酸醛缩酶和NAD依赖性甘油醛-3-磷酸脱氢酶，糖苷水解酶家族61蛋白，可水解纤维素和半纤维素；肌动蛋白和细胞骨架的其他主要成分。DEG富含抗原加工和呈递，光合生物中的碳固定，糖酵解/糖异生，MAPK信号通路，内质网中的蛋白质加工和RNA降解。F. mosseae的接种可以促进大豆的生长发育并提高抗病性。这项研究为进一步研究缓解AMF连续种植大豆带来的挑战的分子机制提供了实验基础。