In cancer cells, fusion genes can produce linear and chimeric fusion-circular RNAs (f-circRNAs), which are functional in gene expression regulation and implicated in malignant transformation, cancer progression, and therapeutic resistance. For specific cancers, proteins encoded by fusion transcripts have been identified as innovative therapeutic targets (e.g., EML4-ALK). Even though RNA sequencing (RNA-Seq) technologies combined with existing bioinformatics approaches have enabled researchers to systematically identify fusion transcripts, specifically detecting f-circRNAs in cells remains challenging owing to their general sparsity and low abundance in cancer cells but also owing to imperfect computational methods.

中文翻译：

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Fcirc：探索融合线性和环状 RNA 的综合管道。

在癌细胞中，融合基因可以产生线性和嵌合的融合环状 RNA（f-circRNA），它们在基因表达调控中起作用，并与恶性转化、癌症进展和治疗抵抗有关。对于特定癌症，融合转录本编码的蛋白质已被确定为创新的治疗靶点（例如，EML4-ALK）。尽管 RNA 测序 (RNA-Seq) 技术与现有的生物信息学方法相结合，使研究人员能够系统地识别融合转录本，但由于 f-circRNA 在癌细胞中普遍稀疏且丰度低，而且计算不完善，因此特异性检测细胞中的 f-circRNA 仍然具有挑战性。方法。