With environmental health and safety becoming a concern for people, supercritical fluid chromatography has been regarded as a “green chemistry” method that gradually has peaked people’s interest. The purpose of this study was to establish a method to screen and quantify the quality markers of Duhuo (DH) through an environmentally friendly ultra-performance convergence chromatography (UPC²) method. Firstly, the fingerprint of different batches of DH samples was established by UPC². Then, the quality markers that can represent the whole information of DH was screened through chemometrics. Finally, the content of the selected quality markers was determined using the UPC² method. From the fingerprint analysis, 24 common peaks were identified. The quantitative analysis results showed that the four compounds could be used as quality markers to represent the overall information and to monitor the quality of DH. This is the first time a traditional Chinese medicine (TCM) fingerprint has been established by UPC² and also the first time to screen the quality markers in DH. The method established in this study can be used for a comprehensive and overall analysis of DH or other natural medicines, and it could serve as an efficient method to contribute to the expansion of the global market for TCMs.

随着人们对环境健康和安全的关注，超临界流体色谱已被视为一种“绿色化学”方法，逐渐引起人们的关注。这项研究的目的是建立一种通过环保的超高性能收敛色谱法（UPC ²）筛选和量化Duhuo（DH）质量标记的方法。首先，通过UPC ²建立了不同批次的DH样品的指纹图谱。然后，通过化学计量学筛选可以代表DH的全部信息的质量标记。最后，使用UPC ²确定所选质量标记的内容方法。通过指纹分析，鉴定出24个共同峰。定量分析结果表明，这四种化合物可用作质量标记，以代表整体信息并监测DH的质量。这是UPC ²首次建立传统中药（TCM）指纹，也是第一次在DH中筛选质量标记。本研究建立的方法可用于DH或其他天然药物的全面，全面分析，并且可作为有效方法来扩展中药的全球市场。