Abstract

In this article, we report a simple, effective, sensitive, non-toxic and environmentally friendly method for the detection of dopamine (DA). This method utilises the property of the self-polymerization of dopamine under alkaline conditions, and gets the concentration of dopamine by measuring the fluorescence intensity of polydopamine (PDA) nanoparticles. And this method only uses NaHCO₃ to start the polymerization process, so that the detection experiment becomes simple and inexpensive. The fluorescence intensities of the mixed solution produced at different reaction times and different concentrations were studied. Finally, a good linear relationship was obtained in the range of 1.0-400 μM with a correlation coefficient of 0.9946 and a detection limit of 0.3 μM. The method has high selectivity and important application value for detecting dopamine-related diseases.

摘要

在本文中，我们报告了一种检测多巴胺（DA）的简单，有效，灵敏，无毒且环境友好的方法。该方法利用了多巴胺在碱性条件下的自聚合特性，并通过测量聚多巴胺（PDA）纳米粒子的荧光强度来获得多巴胺的浓度。而且此方法仅使用NaHCO ₃启动聚合过程，从而使检测实验变得简单而廉价。研究了在不同反应时间和不同浓度下产生的混合溶液的荧光强度。最终，在1.0-400μM的范围内获得了良好的线性关系，相关系数为0.9946，检测极限为0.3μM。该方法具有较高的选择性，对检测多巴胺相关疾病具有重要的应用价值。