A method to determine T-2 and HT-2 toxins, both in feed and food, is described. The method is based on sample clean-up by immunoaffinity columns, followed by triple quadrupole LC-MS/MS determination. It was in-house validated in compliance with the European Union legislation, in the concentration range 5.0–750 µg kg⁻¹ and accredited according to the ISO/IEC 17025 standard, for the scope of official control of feed and food. The method was applied to monitor the contamination levels of T-2 and HT-2 toxins in 105 feed samples, both raw materials and compound feed and 17 cereals and derived products for human consumption, collected in Southern Italy between 2015 and 2019. T-2 toxin was quantified in 32 feed (30.5%) and in 2 food samples (11.8%). HT-2 toxin was quantified in 65 feed (61.9%) and in 3 food (17.6%) samples.

描述了一种测定饲料和食品中T-2和HT-2毒素的方法。该方法基于通过免疫亲和柱净化样品，然后进行三重四极杆LC-MS / MS测定。它是根据欧盟法规进行内部验证的，浓度范围为5.0–750 µg kg ^-1并获得了ISO / IEC 17025标准的认可，用于饲料和食品的官方控制范围。该方法用于监测2015年至2019年在意大利南部收集的105种饲料样品（原材料和复合饲料以及17种谷物和人类食用衍生产品）中T-2和HT-2毒素的污染水平。在32种饲料（30.5％）和2种食品样品（11.8％）中定量了2种毒素。在65种饲料（61.9％）和3种食物（17.6％）样品中定量了HT-2毒素。