At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutant hot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis and hot-1 during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress.

中文翻译：

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两种类型的热胁迫下拟南芥极性甘油脂的定量分析。

在细胞水平上，膜脂质的重塑和热激蛋白的产生是植物抵御热胁迫的两种主要策略。尽管已经单独报道了许多与热应激下的甘油脂和HSPs相关的研究，但是仍需要揭示甘油脂的详细变化以及HSPs在甘油脂变化中的作用。在这项研究中，我们分析了两种类型的热胁迫下野生型拟南芥的甘油脂及其HSP101缺陷型突变型hot-1。我们的结果表明，野生型拟南芥和hot-1中甘油脂的变化非常相似在热应激期间。尽管热适应导致甘油脂略有减少，但是在没有热适应的植物中，热冲击下甘油脂的减少更为严重。在中等温度胁迫下，36：x单半乳糖基二酰基甘油（MGDG）的含量略有增加，而34：6 MGDG和34：4磷脂酰甘油（PG）的含量则严重降低。我们的研究结果表明，热适应可以减少热冲击下甘油脂的降解。通过中等程度的热应激，通过原核途径的甘油脂的合成被严重抑制，而通过真核途径的甘油脂的合成被稍微增强。另外，HSP101对热应激下甘油脂的变化影响很小。