Intramuscular fat (IMF) is one of the major factors determining beef quality. IMF formation is influenced by multiple conditions including genetic background, age and nutrition. In our previous investigation, bta-miR-210 was found to be increased during adipogenesis using miRNA-seq. In this study, we validated the upregulation of bta-miR-210 in platelet-derived growth factor receptor α positive (PDGFRα+) progenitor cells during adipogenic differentiation in vitro. To investigate its role in adipogenesis, bta-miR-210 mimics were introduced into progenitor cells, which resulted in enhanced intracellular lipid accumulation. Accordingly, the expression of adipocyte-specific genes significantly increased in the bta-miR-210 mimic group compared to that in the negative control group (p < 0.01). Dual-luciferase reporter assays revealed that WISP2 is a target of bta-miR-210. WISP2 knockdown enhanced adipogenesis. In conclusion, bta-miR-210 positively regulates the adipogenesis of PDGFRα+ cells derived from bovine fetal muscle by targeting WISP2.

中文翻译：

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bta-miR-210在牛早期肌内脂肪生成中的新机制

肌内脂肪（IMF）是决定牛肉质量的主要因素之一。IMF 的形成受多种条件的影响，包括遗传背景、年龄和营养。在我们之前的研究中，使用 miRNA-seq 发现 bta-miR-210 在脂肪生成过程中增加。在这项研究中，我们验证了在体外成脂分化过程中血小板衍生生长因子受体α阳性（PDGFRα+）祖细胞中 bta-miR-210 的上调。为了研究其在脂肪生成中的作用，将 bta-miR-210 模拟物引入祖细胞，导致细胞内脂质积累增强。因此，与阴性对照组相比，bta-miR-210 模拟组中脂肪细胞特异性基因的表达显着增加（p < 0.01）。双荧光素酶报告基因检测显示 WISP2 是 bta-miR-210 的靶标。WISP2 敲低增强了脂肪生成。总之，bta-miR-210 通过靶向 WISP2 正向调节源自牛胎肌的 PDGFRα+ 细胞的脂肪生成。