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The epidermis-specific cyclin CYCP3;1 is involved in the excess brassinosteroid signaling-inhibited root meristem cell division.
Journal of Integrative Plant Biology ( IF 11.4 ) Pub Date : 2020-05-29 , DOI: 10.1111/jipb.12975
Yuxiao Chen 1 , Shiyong Sun 2 , Xuelu Wang 2
Affiliation  

Cell division is precisely regulated and highly tissue‐specific; studies have suggested that diverse signals in the epidermis, especially the epidermal brassinosteroids (BRs), can regulate root growth. However, the underlying molecular mechanisms that integrate hormonal cues such as BR signaling with other endogenous, tissue‐specific developmental programs to regulate epidermal cell proliferation remain unclear. In this study, we used molecular and biochemical approaches, microscopic imaging and genetic analysis to investigate the function and mechanisms of a P‐type cyclin in root growth regulation. We found that CYCP3;1, specifically expressed in the root meristem epidermis and lateral root cap, can regulate meristem cell division. Mitotic analyses and biochemical studies demonstrated that CYCP3;1 promotes cell division at the G2‐M duration by associating and activating cyclin‐dependent kinase B2‐1 (CDKB2;1). Furthermore, we found that CYCP3;1 expression was inhibited by BR signaling through BRI1‐EMS‐SUPPRESSOR1 (BES1), a positive downstream transcription factor in the BR signaling pathway. These findings not only provide a mechanism of how root epidermal‐specific regulators modulate root growth, but also reveal why the excess of BRs or enhanced BR signaling inhibits cell division in the meristem to negatively regulate root growth.

中文翻译:

表皮特异的细胞周期蛋白CYCP3; 1参与过多的油菜素类固醇信号抑制的根分生组织细胞分裂。

细胞分裂受到精确调节,并且具有高度组织特异性;研究表明,表皮中的多种信号,特别是表皮油菜素类固醇(BRs),可以调节根的生长。但是,尚不清楚将激素信号(例如BR信号转导)与其他内源性,组织特异性的发育程序来调节表皮细胞增殖的潜在分子机制。在这项研究中,我们使用分子和生化方法,显微成像和遗传分析来研究P型细胞周期蛋白在根生长调节中的功能和机制。我们发现CYCP3; 1,专门在根分生组织表皮和侧根帽中表达,可以调节分生组织的细胞分裂。有丝分裂分析和生化研究表明,CYCP3; 1通过关联和激活细胞周期蛋白依赖性激酶B2-1(CDKB2; 1)促进G2-M期的细胞分裂。此外,我们发现CYCP3; 1的表达受到BR信号通过BRI1-EMS-SUPPRESSOR1(BES1)的抑制,BRI1-EMS-SUPPRESSOR1(BR信号通路中的一个积极的下游转录因子)。这些发现不仅提供了根表皮特异性调节剂如何调节根生长的机制,还揭示了为什么过量的BR或增强的BR信号传导抑制分生组织中的细胞分裂,从而负面调节根的生长。
更新日期:2020-05-29
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