Background

Biological procedures for the synthesis of copper nanoparticles (CuNPs) are eco-friendly, cost-effective, and easily scalable as compared to chemical and physical methods.

Methods

In the present study, a simple fungus based synthesis method was used for copper nanoparticles. After morphological and molecular characterization of fungal strains, Aspergillus niger strain STA9 was used for CuNPs synthesis. Particles synthesized by fungi were characterized by UV–visible spectrophotometer, FTIR, and Zetasizer. The MTT anti-cancer, anti-diabetic and antibacterial assays of CuNPs were performed.

Results

The CuNPs were produced at optimized conditions with a size of 500 nm, Z-average 398.2 nm, and 0.246 poly dispersion index. These particles were quantified at 480 nm and FTIR confirmed the existence of OH and -C=C- functional groups. MTT assay revealed that CuNPs have a significant cytotoxic effect against human hepatocellular carcinoma cell lines (Huh-7) with 3.09 μg/ml IC50 value. Alpha-glucosidase inhibition showed that CuNPs have a moderate antidiabetic effect. The agar well antibacterial effect indicated 19, 21, 16, 20, and 17 mm zone of inhibition against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Micrococcus luteus and Bacillus subtilis respectively.

Conclusion

Such biomedical applications of CuNPs reveal the importance of a targeted drug delivery system.

中文翻译：

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一种基于真菌的铜纳米颗粒合成方法，具有抗癌，抗糖尿病和抗菌活性。

背景

与化学和物理方法相比，用于合成铜纳米颗粒（CuNPs）的生物程序环保，成本效益高且易于扩展。

方法

在本研究中，一种简单的基于真菌的合成方法用于铜纳米粒子。在对真菌菌株进行形态和分子表征后，将黑曲霉菌株STA9用于CuNPs合成。紫外可见分光光度计，FTIR和Zetasizer对真菌合成的颗粒进行了表征。进行了MTT对CuNPs的抗癌，抗糖尿病和抗菌测定。

结果

在优化的条件下生产CuNP，尺寸为500 nm，Z平均398.2 nm和0.246的多分散指数。这些颗粒在480 nm处定量，FTIR证实O H和-C = C-官能团的存在。MTT分析表明，CuNP对人肝癌细胞系（Huh-7）具有明显的细胞毒性作用，IC50值为3.09μg/ ml。α-葡萄糖苷酶抑制作用表明CuNP具有中等程度的抗糖尿病作用。琼脂孔的抗菌作用表明分别对大肠杆菌，金黄色葡萄球菌，肺炎克雷伯菌，黄体微球菌和枯草芽孢杆菌有19、21、16、20和17 mm的抑制区。

结论

CuNPs的这种生物医学应用揭示了靶向药物递送系统的重要性。