This research investigated the use of high-pressure processing (HPP) for inactivating vegetative pathogens and spoilage microbiota in fresh unfiltered coconut water (Cocos nucifera L) from nuts obtained from Florida and frozen CW from Brazil with pH >5.0 and storage at 4 °C. Additionally, CW was evaluated to determine if it supported the growth and toxin production of Clostridium botulinum with or without the use of HPP when stored at refrigeration temperatures. Samples of fresh unfiltered CW were inoculated to 5.5 to 6.5 logs/mL with multiple strain cocktails of E. coli O157:H7, Salmonella spp. and Listeria monocytogenes and HPP at 593 MPa for 3 min at 4 °C. HPP and inoculated non-HPP controls were stored at 4 °C for 54 and 75 days for Florida CW and Brazil CW, respectively. Results of analyses showed HPP samples with <1 CFU/mL and no detection (negative/25 mL) with enrichment procedures for the 3 inoculated pathogens for all analyses. The non-HPP control samples did not show growth of the pathogens but a gradual decrease in levels to ca. 3-Logs/mL by day 54 in the fresh Florida CW and similarly in frozen Brazil CW by Day 75. Microbial spoilage of uninoculated samples was evaluated for normal spoilage microbiota through 120 days storage at 4 °C. Microbial counts remained at ca. 2-logs with no detectable signs of spoilage for HPP samples through 120 d. The non-HPP control samples spoiled within 2 weeks of storage at 4 °C with gas production, cloudiness, and off-odors. To evaluate if CW supports the growth and toxin production of C. botulinum, samples of unfiltered and filtered (0.2 μm) CW were inoculated with either proteolytic or non-proteolytic C. botulinum spores at 2 log CFU/mL that were processed at 593 MPa for 3 min and stored at 4 °C and 10 °C for 45 days. Inoculated positive and non-inoculated negative controls were prepared and stored as the HPP treated and non-HPP samples. No growth of C. botulinum or toxin production was detected in either the unfiltered or filtered CW regardless if products were HPP treated or not. All inoculated samples with C. botulinum spores were enriched at Day-45 in PYGS media to determine the viability of the inoculated spores at the end of shelf-life and screened for C. botulinum toxins. In all samples, C. botulinum toxin Types A, B and E were detected indicating spores were viable throughout the storage. Type F toxin was not detected possibly due to inherent conditions in the samples that may affected toxin screening.

这项研究调查了使用高压处理（HPP）灭活来自佛罗里达州的坚果和巴西冷冻的CW（pH> 5.0）的新鲜未经过滤的椰子水（Cocos nucifera L）中的营养菌和腐败菌群，并在4°C下存储。此外，对CW进行了评估，以确定在冷藏温度下存储或不使用HPP时，CW是否支持肉毒梭菌的生长和毒素产生。新鲜的未经过滤的连续波样品用多种E菌混合液接种至5.5至6.5 log / mL 。大肠杆菌O157：H7，沙门氏菌属。和单核细胞增生李斯特菌和HPP在593 MPa下于4°C放置3分钟。HPP和接种的非HPP对照分别在佛罗里达CW和巴西CW中分别于4°C存放54天和75天。分析结果显示HPP样品的<1 CFU / mL，未检测到（阴性/ 25 mL），并且对所有3种接种病原体均进行了富集程序。非HPP对照样品未显示病原体生长，但水平逐渐降低至约。在第54天时，新鲜佛罗里达州的CW中的细菌含量为3-Logs / mL，在第75天时，在冷冻的巴西CW中也是如此。在4°C下储存120天，对未接种样品的微生物腐败度进行了正常腐败菌的评估。微生物数量保持在约。直到120天，HPP样品均没有2记录的变质迹象。非HPP对照样品在4°C下储存2周内变质，产生气体，浑浊，和异味。评估CW是否支持其生长和毒素产生Ç。在肉毒杆菌中，未过滤和已过滤（0.2μm）的CW样品分别用蛋白水解或非蛋白水解C接种。以2 log CFU / mL的肉毒杆菌孢子在593 MPa下处理3分钟，并在4°C和10°C下储存45天。制备了接种的阳性和未接种的阴性对照，并作为HPP处理和非HPP样品保存。没有C的增长。无论产品是否经过HPP处理，都可以在未过滤或过滤的化学废物中检测到肉毒杆菌或毒素的产生。所有接种样品Ç。肉毒杆菌孢子在日间45 PYGS媒体富集以确定接种孢子的保质期结束时的存活力，并筛选Ç。肉毒杆菌毒素。在所有样本中，C。检测到A，B和E型肉毒杆菌毒素，表明孢子在整个储存过程中都是有活力的。未检测到F型毒素，可能是由于样品中的固有条件可能会影响毒素筛选。