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Economical lipid production from crude glycerol using Rhodosporidiobolus fluvialis DMKU-RK253 in a two-stage cultivation under non-sterile conditions
Biomass & Bioenergy ( IF 6 ) Pub Date : 2020-05-29 , DOI: 10.1016/j.biombioe.2020.105597
Pirapan Polburee , Savitree Limtong

To enhance the microbial lipid production through Rhodosporidiobolus fluvialis DMKU-RK253, a two-stage cultivation process with a focus on cost reduction was developed. Low-cost raw materials, which are biodiesel-derived crude glycerol and monosodium glutamate were utilized to produce microbial lipids. A two-stage cultivation process was performed under non-sterile cultivation, and a response surface methodology was used to determine the effects of the interaction among factors in the first and second stages of cultivation while using shaking flask cultivation. In the first stage of cultivation, cells were cultivated in a nitrogen-rich medium for cell mass production. The optimal crude glycerol (30 g/L) and monosodium glutamate (5 g/L) concentrations, and the inoculum age (96 h), were found to improve cell mass production. In the second stage, a non-sterile crude glycerol solution was used to enhance lipid production without any aseptic techniques. An optimal crude glycerol concentration of 57 g/L with an initial pH of 7 and an initial cell concentration of optical density at 600 nm of 20 resulted in a high lipid production of 27.81 ± 1.86 g/L and maximum cell mass of 39.14 ± 0.08 g/L. These high lipid and cell mass concentrations consequent to achieve a high lipid content of 71.42% of the dry cell mass, a lipid yield of 0.34 g/g glycerol and a cell mass yield of 0.48 g/g glycerol. The low-cost raw materials were estimated to be US$0.80/kg for microbial lipid production. Therefore, a potential condition for the cultivation process of yeast that produces a high amount of inexpensive lipids has been demonstrated.



中文翻译:

在非无菌条件下分两步培养,使用Rhodosporidiobolus fluvialis DMKU-RK253从粗甘油中经济生产脂质

通过河流红假单胞菌提高微生物脂质的产生开发了以降低成本为重点的两阶段栽培工艺DMKU-RK253。低成本原料是生物柴油衍生的粗制甘油和味精,可用于生产微生物脂质。在非无菌培养下进行两阶段培养过程,并使用响应面分析法确定第一阶段和第二阶段因素的相互作用,同时使用摇瓶培养。在培养的第一阶段,将细胞在富氮培养基中培养以大量生产细胞。发现最佳的粗甘油(30 g / L)和谷氨酸钠(5 g / L)的浓度以及接种物的年龄(96 h)可以提高细胞的产量。在第二阶段 使用非无菌的粗甘油溶液无需任何无菌技术即可提高脂质产量。最佳粗甘油浓度为57 g / L,初始pH为7,初始细胞在600 nm处的光密度为20,导致高脂质产量为27.81±1.86 g / L,最大细胞质量为39.14±0.08克/升 这些高的脂质和细胞质量浓度因此实现了干细胞质量的71.42%的高脂质含量,0.34 g / g甘油的脂质产量和0.48 g / g甘油的细胞产量。用于生产微生物脂质的低成本原料估计为0.80美元/千克。因此,已经证明了产生大量廉价脂质的酵母培养过程的潜在条件。最佳粗甘油浓度为57 g / L,初始pH为7,初始细胞在600 nm处的光密度为20,导致高脂质产量为27.81±1.86 g / L,最大细胞质量为39.14±0.08克/升 这些高的脂质和细胞质量浓度因此实现了干细胞质量的71.42%的高脂质含量,0.34 g / g甘油的脂质产量和0.48 g / g甘油的细胞产量。用于生产微生物脂质的低成本原料估计为0.80美元/千克。因此,已经证明了产生大量廉价脂质的酵母培养过程的潜在条件。最佳粗甘油浓度为57 g / L,初始pH为7,初始细胞在600 nm处的光密度为20,导致高脂质产量为27.81±1.86 g / L,最大细胞质量为39.14±0.08克/升 这些高的脂质和细胞质量浓度因此实现了干细胞质量的71.42%的高脂质含量,0.34 g / g甘油的脂质产量和0.48 g / g甘油的细胞产量。用于生产微生物脂质的低成本原料估计为0.80美元/千克。因此,已经证明了产生大量廉价脂质的酵母培养过程的潜在条件。08克/升。这些高的脂质和细胞质量浓度因此实现了干细胞质量的71.42%的高脂质含量,0.34 g / g甘油的脂质产量和0.48 g / g甘油的细胞产量。用于生产微生物脂质的低成本原料估计为每公斤0.80美元。因此,已经证明了产生大量廉价脂质的酵母培养过程的潜在条件。08克/升。这些高的脂质和细胞质量浓度因此实现了干细胞质量的71.42%的高脂质含量,0.34 g / g甘油的脂质产量和0.48 g / g甘油的细胞产量。用于生产微生物脂质的低成本原料估计为0.80美元/千克。因此,已经证明了产生大量廉价脂质的酵母培养过程的潜在条件。

更新日期:2020-05-29
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