Mid1ip1 plays important roles in lipogenesis and microtubule stabilization, but little relevant information has been reported in fish. In the present study, mid1ip1 cDNA of tiger puffer was cloned and characterized, and its expression in response to dietary bile acid and lipid levels was investigated following a feeding trial. Experimental diets with suitable (8.5 %) or high lipid level (12.5 %), with or without bile acid supplementation (0.02 % and 0.10 % in the suitable-lipid diet; 0.02 % in the high-lipid diet) were used in the feeding trial. The tiger puffer Mid1ip1, which is putative Mid1ip1 subtype B, shared moderate identity to its known orthologs of other teleost and much lower identity to human Mid1ip1. Mid1ip1 had high transcription in the muscle, skin, eye, and brain, but low expression levels in the liver, intestine, and spleen. The high dietary bile acid level (0.1 %) significantly (P < 0.05) reduced the hepatic mRNA expression of mid1ip1, but unexpectedly the dietary lipid level did not significantly (P > 0.05) affect the gene expression. Significantly positive correlation (R² = 0.812, P = 0.000) was observed between hepatic mRNA expression of mid1ip1 and tubulin-α, but not between the gene expression of mid1ip1 and acetyl-CoA carboxylase β (R² = -0.434, P = 0.106), in response to dietary treatments, indicating the potential roles of this Mid1ip1 subtype in microtubule stabilization rather than in lipogenesis. To our knowledge, this is the first study investigating the regulation of fish mid1ip1 expression by dietary nutrients.

Mid1ip1在脂肪生成和微管稳定中起着重要作用，但是鲜有相关信息报道。在本研究中，mid1ip1克隆并鉴定了老虎河豚的cDNA，并在喂养试验后研究了其对饮食中胆汁酸和脂质水平的响应表达。饲喂中添加（8.5％）或高脂（12.5％）或不添加胆汁酸的实验性饲料（在合适的脂质饮食中为0.02％和0.10％；在高脂饮食中为0.02％）试用。老虎河豚Mid1ip1是推定的Mid1ip1亚型B，与其他硬骨鱼的直系同源基因具有中等身份，而与人类Mid1ip1的同源性低得多。Mid1ip1在肌肉，皮肤，眼睛和大脑中具有高转录，而在肝，肠和脾中却低表达。饮食中较高的胆汁酸水平（0.1％）显着（P  <0.05）降低了mid1ip1肝脏mRNA表达但出乎意料的是，饮食中的血脂水平并未显着影响（P  > 0.05）基因表达。mid1ip1和微管蛋白-α的 肝mRNA表达之间存在显着正相关（R ²  = 0.812，P = 0.000），但mid1ip1的基因表达与乙酰辅酶A羧化酶β之间无显着正相关（R ² = -0.434，P  = 0.106） ），这是对饮食疗法的回应，表明该Mid1ip1亚型在微管稳定而不是脂肪形成中的潜在作用。据我们所知，这是第一项研究饮食营养对鱼mid1ip1表达的调节的研究。