Key Message

The study has facilitated important insights into the regulatory networks involved in flower development in chrysanthemum (Asteraceae), and is informative with respect to the mechanism of flower shape determination.

Abstract

Chrysanthemum morifolium, valued as an ornamental species given the diversity of its inflorescence form, is viewed as a model for understanding flower development in the Asteraceae. Yet, the underlying regulatory networks remain largely unexplored. Here, a transcriptomic survey of the Chrysanthemum morifolium variety ‘Jinba’ was undertaken to uncover the global gene expression profiles and identify the modules of co-transcribed genes associated with flower development. The weighted gene coexpression network analysis revealed important networks and hub genes including ray floret petals-specific coexpression network, disc floret petals-specific network, B and E class genes involved network and CYC2 genes network. Three ray floret petal-specific hub genes were also strongly transcribed in the ray florets of a selection of six diverse varieties and especially so in those which form ligulate ray floret petals. CmCYC2c was strongly transcribed in the distal and lateral regions of the ray floret petals, and also, along with CmCYC2d, in the tubular ray florets. Furthermore, CmOFP, belonging to the family of ovate proteins, was identified in the CYC2 genes network. CmOFP can interact with CmCYC2d that physically interact with CmCYC2c. This work provides important insights into the regulatory networks involved in flower development in chrysanthemum, and is informative with respect to the mechanistic basis of the regulation of flower shape.

中文翻译：

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菊花发育的核心调控网络和枢纽基因。

关键信息

该研究有助于深入了解菊花（菊科）花卉发育所涉及的调控网络，并为花形确定的机制提供信息。

抽象的

鉴于其花序形式的多样性，菊花被视为观赏物种，被视为了解菊科花卉发育的模型。然而，潜在的监管网络在很大程度上仍未被探索。在这里，对菊花品种“Jinba”进行了转录组学调查，以揭示全球基因表达谱并确定与花发育相关的共转录基因模块。加权基因共表达网络分析揭示了重要的网络和枢纽基因，包括射线小花花瓣特异性共表达网络、圆盘小花花瓣特异性网络、B和E类基因参与网络和CYC2基因网络。三个射线小花花瓣特异性中枢基因也在六种不同品种的射线小花中被强烈转录，尤其是在形成舌状射线小花花瓣的那些中。CmCYC2c在射线小花花瓣的远端和外侧区域被强烈转录，并且与CmCYC2d一起在管状射线小花中被强烈转录。此外，在CYC2基因网络中鉴定出属于卵形蛋白家族的CmOFP。CmOFP 可以与与 CmCYC2c 物理相互作用的 CmCYC2d 相互作用。这项工作为菊花发育中涉及的调控网络提供了重要的见解，并为花形调控的机制基础提供了信息。