The incidence of resistance to antifungal agents for dermatophytes is increasing, but most of the methods currently available to test the antifungal susceptibility of Microsporum canis still require standardization. The aims of this study were: (i) to evaluate the antifungal susceptibility of M. canis strains recovered from animals to ketoconazole (KTZ), fluconazole (FLZ) and itraconazole (ITZ) using a modified CLSI broth microdilution (CLSI M38-A2-BMD) and the E-test® protocols and (ii) to estimate the agreement between the methods. Tentative azole epidemiological cutoff values (ECVs) were also proposed in order to interpret the results of in vitro susceptibility tests and to establish the agreement between the E-test and CLSI BMD methods. A total of forty clinical M. canis strains from animals with skin lesions were tested, and the essential (EA) and categorical agreement (CA) between the two methods were determined. KTZ displayed the lowest MIC values, while ITZ and FLZ the highest. The ECV for KTZ and ITZ were 4 μg/ml, while those of FLZ was 64 μg/ml. Based on ECVs, about 88% of M. canis strains were susceptible to all azoles being a cross-resistance with ITZ-FLZ registered for one strain. A total of five M. canis strains showed MIC > ECV for FLZ using CLSI, while one strain showed MIC > ECV for ITZ using both tests. KTZ, ITZ and FLZ showed EA ranging from 92.5 to 95%, for all azoles and CA > 97% except for FLZ (87.5%). The good CA between the E-test and the CLSI BMD provides evidence of the reliability of the former method to test the antifungal susceptibility of M. canis for ITZ and KTZ and not for FLZ.

对皮肤癣菌的抗真菌剂耐药性的发生率正在增加，但是目前可用于测试犬小孢子菌抗真菌药性的大多数方法仍需要标准化。本研究的目的在于：（ⅰ）来评价的抗真菌敏感性犬小孢霉的菌株从动物中回收对酮康唑使用改良的CLSI肉汤微量稀释（KTZ），氟康唑（FLZ）和伊曲康唑（ITZ）（CLSI M38-A2- BMD）和E-test®协议，以及（ii）估算方法之间的一致性。还提出了暂定的唑流行病学临界值（ECV），以解释体外药敏试验的结果，并建立E检验和CLSI BMD方法之间的一致性。共有40个临床M。测试了来自具有皮肤损伤动物的犬的canis菌株，并确定了两种方法之间的基本（EA）和分类一致性（CA）。KTZ显示最低的MIC值，而ITZ和FLZ显示最高的MIC。KTZ和ITZ的ECV为4μg/ ml，而FLZ的ECV为64μg/ ml。基于ECV，约88％的犬莫尔氏菌菌株易感所有与对一种菌株注册的ITZ-FLZ有交叉耐药性的唑类。总共五个犬莫斯犬使用CLSI的FLZ菌株显示MIC> ECV，而使用ITZ的两种菌株显示MIC> ECV。KTZ，ITZ和FLZ的EA范围从92.5％到95％，除FLZ（87.5％）以外，CA的CA均> 97％。E测试和CLSI BMD之间的良好CA证明了前一种方法的可靠性，该方法可以测试犬莫氏杆菌对ITZ和KTZ而非FLZ的抗真菌药性。