Novel enzymatically hardened tetracalcium phosphate/monetite cements were prepared applying phytic acid/phytase (PHYT/F3P) mixture as hardening liquid after dissolving in acetic acid solution (CX cement). Properties of the cements were compared with classic cement hardened with 2% NaH₂PO₄ (C cement) and cement hardened with acetic acid solution (CAC cement) only. In the microstructure of CX cement, columnar growth of hydroxyapatite particles was found in the form of walls around hydroxyapatite agglomerates originated from tetracalcium phosphate which were mutually separated by a material depleted low density zone. Wet compressive strengths (CS) of all cements were practically identical contrary to about 30% higher dry CS’s of CX and CAC cements due to specific microstructure. It was verified noncytotoxic character of CX cement extracts and positive effect of CX cement on ALP activity and cell behavior during cultivation. The final Ca/P molar ratio and setting time of cement were effectively controlled by the amount of phytic acid and the change in PHYT/F3P mass ratio, or reaction time in hardening liquid, respectively.

将植酸/植酸酶（PHYT / F3P）混合物作为硬化液溶解在乙酸溶液（CX水泥）中后，制备了新型的酶促硬化磷酸四钙/透钙粘土水泥。将水泥的性能与用2％NaH ₂ PO ₄硬化的经典水泥进行比较（C水泥）和仅用乙酸溶液硬化的水泥（CAC水泥）。在CX水泥的微观结构中，发现羟基磷灰石颗粒的柱状生长形式为源自磷酸四钙的羟基磷灰石附聚物周围的壁，这些壁通过材料贫乏的低密度区相互分隔。由于特定的微结构，所有水泥的湿抗压强度（CS）实际上是相同的，而CX和CAC水泥的干CS则高约30％。证实了CX水泥提取物的无细胞毒性特征，以及CX水泥对培养过程中ALP活性和细胞行为的积极影响。通过植酸的量和PHYT / F3P质量比的变化或硬化液中的反应时间，可以有效地控制水泥的最终Ca / P摩尔比和凝固时间。