Drugs of abuse can be investigated in dried blood spot (DBS) samples to evaluate the intake of psychoactive drugs. We have developed the validated liquid chromatography–tandem mass spectrometry method to determine drug of abuse in whole blood with DBS. The mass spectrometer was performed using multiple reaction monitoring via positive electro-spray ionization. The analysis procedure was applied to real positive blood samples of the 59 cases brought to Forensic Toxicology Laboratory in Faculty of Medicine of Cukurova University by law enforcement agencies. The method based on liquid–liquid extraction and liquid chromatography tandem mass spectrometry was fully validated according to international guidelines and then applied to authentic positive blood samples. Overall, polydrug usage was determined in 24 real samples and one or more psychoactive drugs were found positive in 51 of 59 real samples, which were found to be positive via performing routine whole blood analysis and selected for DBS analysis. Our method developed could applicate as an alternative to conventional analytical and sampling techniques and respond to some of the present issues concerning the different forensic toxicological investigations.

中文翻译：

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LC-MS/MS 方法测定干血斑中的违禁药物：真实样品的验证和应用

可以在干血斑 (DBS) 样本中调查滥用药物，以评估精神活性药物的摄入量。我们开发了经过验证的液相色谱-串联质谱法，可使用 DBS 测定全血中的滥用药物。质谱仪是通过正电喷雾电离使用多反应监测进行的。该分析程序适用于执法机构带到库库罗瓦大学医学院法医毒理学实验室的 59 例真实阳性血液样本。基于液液萃取和液相色谱串联质谱的方法根据国际指南进行了充分验证，然后应用于真实的阳性血样。全面的，在 24 个实际样本中确定了多种药物使用情况，在 59 个实际样本中的 51 个中发现一种或多种精神药物呈阳性，这些样本通过常规全血分析发现呈阳性并被选择用于 DBS 分析。我们开发的方法可以作为传统分析和采样技术的替代方法，并应对有关不同法医毒理学调查的一些当前问题。