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Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron.
Genes & Development ( IF 10.5 ) Pub Date : 2020-07-01 , DOI: 10.1101/gad.338194.120
Jihyun Irizarry 1 , James McGehee 1 , Goheun Kim 2 , David Stein 2 , Angelike Stathopoulos 1
Affiliation  

Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets.

中文翻译:

使用光诱导 degron 揭示了在 Dorsal 下游发挥作用的依赖于扭曲的棘轮。

分级转录因子是胚胎模式的关键调节因子,但它们的作用是否会随时间发生变化尚不清楚。光调节蛋白质降解系统用于测定转录因子 Dorsal 在果蝇胚胎的背腹轴模式中的时间依赖性。令人惊讶的是,高阈值目标基因蜗牛只需要在 Dorsal 水平达到峰值时尽早提供 Dorsal 输入,而不需要在后期提供。相反,晚期蜗牛表达可以通过 Twist 转录因子的作用得到支持,特别是通过一种增强子sna.distal。这项研究表明,某些 Dorsal 目标和下游响应不需要连续输入,例如扭曲, 起到分子棘轮的作用。
更新日期:2020-07-01
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