Acibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial diseases. However, resistant strains have already been found. Therefore, there is an increasing demand for sustainable new disease control strategies. We investigated the ASM disease control effect against Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight on Japanese radish. In this study, we demonstrated that ASM effectively suppressed Pcal disease symptom development associated with reduced bacterial populations on Japanese radish leaves. Interestingly, we also demonstrated that ASM activated systemic acquired resistance (SAR), including stomatal-based defense, not only on ASM treated leaves, but also on untreated upper and lower leaves. Reactive oxidative species (ROS) are essential second messengers in stomatal-based defense. We found that ASM induced stomatal closure by inducing ROS production through peroxidase. These results indicate that stomatal closure induced by ASM treatment is effective for preventing Pcal pathogen invasion into plants, and in turn reduction of disease development.

中文翻译：

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Acibenzolar-S-methyl通过诱导过氧化物酶依赖性活性氧的产生，系统地激活日本萝卜中的气孔基防御

Acibenzolar-S-methyl（ASM）是一种众所周知的植物活化剂，它是水杨酸（SA）的合成类似物。近来，铜杀真菌剂和抗生素是控制细菌疾病的主要策略。但是，已经发现了抗性菌株。因此，对可持续的新疾病控制策略的需求日益增长。我们调查了ASM对大麻假单胞菌pv的疾病控制作用。alisalensis（Pcal是），这将导致在日本萝卜细菌性疫病。在这项研究中，我们证明了ASM有效抑制Pcal萝卜叶片上细菌种群减少与疾病症状发展有关。有趣的是，我们还证明了ASM不仅在ASM处理的叶子上而且在未处理的上部和下部叶子上都激活了系统获得性抗性（SAR），包括基于气孔的防御。活性氧化物质（ROS）是基于气孔的防御中必不可少的第二信使。我们发现ASM通过通过过氧化物酶诱导ROS产生来诱导气孔关闭。这些结果表明，通过ASM处理诱导的气孔关闭对于防止Pcal病原体侵入植物是有效的，从而减少了疾病的发展。