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Molecular detection of pathogens in ticks and fleas collected from companion dogs and cats in East and Southeast Asia
bioRxiv - Molecular Biology Pub Date : 2020-05-27 , DOI: 10.1101/2020.05.27.118554
Viet-Linh Nguyen , Vito Colella , Grazia Greco , Fang Fang , Wisnu Nurcahyo , Upik Kesumawati Hadi , Virginia Venturina , Kenneth Boon Yew Tong , Yi-Lun Tsai , Piyanan Taweethavonsawat , Saruda Tiwananthagorn , Sahatchai Tangtrongsup , Thong Quang Le , Khanh Linh Bui , Thom Do , Malaika Watanabe , Puteri Azaziah Megat Abd Rani , Filipe Dantas-Torres , Lenaig Halos , Frederic Beugnet , Domenico Otranto

Ticks and fleas are considered amongst the most important arthropod vectors of medical and veterinary concern due to their ability to transmit pathogens to a range of animal species including dogs, cats and humans. By sharing a common environment with humans, companion animal-associated parasitic arthropods may potentially transmit zoonotic vector-borne pathogens (VBPs). This study aimed to molecularly detect pathogens from ticks ( n = 392) and fleas ( n = 248) collected from companion dogs and cats in East and Southeast Asia. Of the 392 ticks tested, 37 (9.4%) scored positive for at least one pathogen with Hepatozoon canis being the most prevalent (5.4%), followed by Ehrlichia canis (1.8%), Babesia vogeli (1%), Anaplasma platys (0.8%) and Rickettsia spp. (1%) [including Rickettsia sp. (0.5%), Rickettsia asembonensis (0.3%), Rickettsia felis (0.3%)]. Out of 248 fleas tested, 106 (42.7%) were harboring at least one pathogen with R. felis being the most common (19.4%), followed by Bartonella spp. (16.5%), Rickettsia asembonensis (10.9%) and Candidatus Rickettsia senegalensis (0.4%). Furthermore, 35 Rh ipicephalus sanguineus sensu lato ticks were subjected to phylogenetic analysis, of which 34 ticks belonged to the tropical and only one belonged to the temperate lineage ( Rh. sanguineus sensu stricto). Our data reveals the circulation of different VBPs in ticks and fleas of dogs and cats from Asia, including zoonotic agents, which may represent a potential risk to animal and human health.

中文翻译:

从东亚和东南亚的猫和狗身上采集的lea和跳蚤中的病原体的分子检测

和跳蚤被认为是医学和兽医学中最重要的节肢动物载体,因为它们具有将病原体传播给包括狗,猫和人在内的多种动物的能力。通过与人类共享共同的环境,与动物相关的寄生节肢动物可能会传播人畜共患病的媒介传播病原体(VBP)。这项研究旨在从东亚和东南亚的犬只和猫的collected(n = 392)和跳蚤(n = 248)中分子检测病原体。在测试的392个s中,至少一种病原体的阳性率为37(9.4%),其中犬肝最普遍(5.4%),其次是犬埃里希氏菌(1.8%),贝贝斯贝格斯虫(1%),板状浆虫(0.8) %)和立克次体属。(1%)[包括立克次体sp。(0.5%),阿森伯立克次体(0.3%),猫立克次体(0。3%)]。在测试的248条跳蚤中,有106种(42.7%)带有至少一种病原体,其中以R. felis最常见(19.4%),其次是Bartonella spp。(16.5%),阿肯色立克次体(10.9%)和塞内加尔立假丝酵母(0.4%)。此外,还对35个i猴Ripicephalus sanguineus sensu lato进行了系统发育分析,其中34个ed属于热带,而只有一个属于温带谱系(Rh。sanguineus sensu stricto)。我们的数据揭示了来自亚洲的狗和猫的虱子和跳蚤中不同VBP的循环,包括人畜共患病因素,这可能对动物和人类健康构成潜在风险。9%)和塞内加尔立克氏菌(0.4%)。此外,还对35个i猴Ripicephalus sanguineus sensu lato进行了系统发育分析,其中34个ed属于热带,而只有一个属于温带谱系(Rh。sanguineus sensu stricto)。我们的数据揭示了来自亚洲的狗和猫的虱子和跳蚤中不同VBP的循环,包括人畜共患病因素,这可能对动物和人类健康构成潜在风险。9%)和塞内加尔立克氏菌(0.4%)。此外,还对35个i猴Ripicephalus sanguineus sensu lato进行了系统发育分析,其中34个ed属于热带,而只有一个属于温带谱系(Rh。sanguineus sensu stricto)。我们的数据揭示了来自亚洲的狗和猫的虱子和跳蚤中不同VBP的循环,包括人畜共患病因素,这可能对动物和人类健康构成潜在风险。
更新日期:2020-05-27
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