Rapid cryo-preservation of biological specimens is the gold standard for visualising cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo electron microscopy or cryo soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly open source Python control software. Therefore, cryoSIM democratises the ability to detect molecules using super-resolution fluorescence imaging of cryo-preserved specimens for correlation with their cellular ultrastructure.

中文翻译：

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CryoSIM：用于相关超结构成像的超高分辨率3D结构化照明低温荧光显微镜

快速冷冻保存生物标本是在其真实结构背景下可视化细胞结构的金标准。但是，当前的商用低温荧光显微镜仅限于低分辨率。为了填补这一空白，我们开发了cryoSIM，这是一种用于3D超分辨率荧光冷冻成像的显微镜，可与冷冻电子显微镜或冷冻软X射线断层扫描相关。我们提供完整的说明，主要用于从现成的组件和可访问的，用户友好的开源Python控制软件中复制仪器。因此，cryoSIM使使用冷冻保存的标本的超分辨率荧光成像及其与细胞超微结构相关的分子检测能力更加民主化。