The methane production and the microbial community dynamics of thermophilic anaerobic co-digestion (AD) of corn stover, swine manure and effluent were conducted at total solid (TS) content of 5%, 10% and 15%, the carbon to nitrogen ratio (C/N) of 20, 30 and 40 and the effluent volumetric percentage (EVP) of 20%, 40% and 60%. For batches with 5% TS, the highest methane yield of 238.5–283.1 mL g⁻¹ volatile solid (VS) and the specific methane productivity of 138.5–152.2 mL g⁻¹ initial VS were obtained at the C/N ratios of 20 and 30. For the mixtures with 10% and 15% TS, the highest methane yield was 341.9 mL g⁻¹ VS and 351.2 mL g⁻¹ VS, respectively, when the C/N ratio of 20% and 60% EVP conditions were maintained. Co-digestion of swine manure with corn stover caused an obvious shift in microbial population, in which the archaeal population changed from 0.3% to 2.8% and the bacterial community changed from 97.2% to 99.7%. The experimental batches with the highest relative abundance of the archaeal population (2.00% of total microbial population for 5% TS, 1.74% for 10% TS and 2.76% for 15% TS) had the highest rate of methanogenesis subsequently enhancing methane production (283.08 mL g⁻¹ VS for 5% TS, 341.91 mL g⁻¹ VS for 10% TS and 351.23 mL g⁻¹ VS for 15% TS). The results of microbiome analysis enabled understanding the key populations in biomethane generation.

在总固形物（TS）含量为5％，10％和15％，碳氮比为（TS）的条件下，进行了玉米秸秆，猪粪和废水中甲烷厌氧消化过程（AD）的甲烷生产和微生物群落动态。 C / N）为20、30和40，流出物体积百分比（EVP）为20％，40％和60％。对于含5％TS的批次，在C / N比为20和20时，最高甲烷产量为238.5–283.1 mL g ^-1挥发性固体（VS），甲烷比生产率为138.5–152.2 mL g ^-1初始VS。30.对于具有10％和15％TS的混合物，最高甲烷产量为341.9 mL g ^-1 VS和351.2 mL g ^-1当维持C / N比为20％和60％EVP的条件下分别为VS。猪粪与玉米秸秆的共同消化导致微生物种群发生明显变化，其中古细菌种群从0.3％变为2.8％，细菌群落从97.2％变为99.7％。在古细菌种群中相对丰度最高的实验批次（5％TS占总微生物种群的2.00％，10％TS占1.74％，15％TS的占2.76％）具有最高的产甲烷率，从而提高了甲烷产量（283.08）毫升克^-1 VS为5％TS，341.91毫升克^-1 VS 10个％TS和351.23毫升克^-1 VS为15％TS）。微生物组分析的结果有助于了解生物甲烷生成中的关键种群。