Abstract

Encapsulation techniques and materials, explored for addressing compromised probiotic gut survival, report significant production losses resulting in <10% entrapment. Presently, we report three-time enhanced entrapment (30 ± 1.2%) of Lactobacillus acidophilus (LAB) in calcium-alginate beads, by modifying process parameters and employing polyethylene glycol (PEG). Water-loving, viscolysing and osmotic-building effects of PEG create numerous, fine voids in the alginate gel allowing efficient diffusion of crosslinking calcium ions, resulting in less leaky beads. Eudragit S100 overcoat improved LAB survival by 690 times in simulated GIT stresses.

In DMH-DSS induced colitis and precancerous lesions in rats, while free LAB failed to show any protection, pharmabiotic beads significantly (p < .05) reduced lipid peroxidation, increased antioxidant levels; decreased serum inflammatory burden; downregulated COX-2, iNOS, and c-Myc expression; elevated levels of the selected gut bacteria and SCFAs especially butyrate, all of which add up to antioxidant, anti-inflammatory, balanced gut biota, and ultimately anticancer effects.

摘要

为解决益生菌肠道存活率受损而探索的封装技术和材料报告了显着的生产损失，导致 <10% 的截留。目前，我们通过修改工艺参数和使用聚乙二醇 (PEG)报告了三倍增强的嗜酸乳杆菌(LAB) 在海藻酸钙珠中的截留 (30 ± 1.2% )。PEG 的亲水性、粘稠性和渗透性构建作用在藻酸盐凝胶中产生大量细小空隙，允许交联钙离子有效扩散，从而减少泄漏的珠子。Eudragit S100 外涂层在模拟 GIT 压力下将 LAB 存活率提高了 690 倍。

在 DMH-DSS 诱导的大鼠结肠炎和癌前病变中，虽然游离 LAB 没有表现出任何保护作用，但药丸显着 ( p  < .05) 降低脂质过氧化，增加抗氧化水平；降低血清炎症负荷；下调COX-2、iNOS和c-Myc表达；选定的肠道细菌和 SCFA 的水平升高，尤其是丁酸盐，所有这些加起来都具有抗氧化、抗炎、平衡肠道生物群，并最终具有抗癌作用。