Mixed fermentation with different microorganisms can facilitate fermentation metabolism and increase the low molecular metabolites accumulation, thereby enhancing the bioactive activity. In this study, we evaluated the immune‐stimulating activities of Spirulina platensis and different extracts from fermented S. platensis (FS) by mixed probiotics in vitro, by measuring the proliferation and Th1/Th2 immunomodulatory potential on murine primary splenic lymphocytes. The results showed that mixed fermentation enhanced the immunomodulatory activity of S. platensis with higher lymphocyte proliferation compared with non‐fermented S. platensis (NFS). Notably, the low molecular weight (<3 kDa) peptide fraction from fermented S. platensis (L‐PFS), especially at 40 μg/ml, presented the strongest activity in promoting lymphocytes proliferation and modulating cytokines (IL‐2 and IL‐10) secretion. Meanwhile, L‐PFS enhanced the relative mRNA expression of Th1 cytokine (IFN‐γ) and Th2 cytokine (IL‐4), along with inhibiting the relative mRNA expression of Th1 cytokines (IL‐2 and TNF‐α) and Th2 cytokine (IL‐10) compared with Concanavalin A‐treated lymphocytes.

中文翻译：

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混合益生菌对螺旋藻发酵螺旋藻多肽级分的免疫增强潜力。

与不同微生物混合发酵可以促进发酵代谢并增加低分子代谢物的积累，从而增强生物活性。在这项研究中，我们通过测量鼠原代脾淋巴细胞的增殖和Th1 / Th2免疫调节潜力，评估了混合螺旋菌在体外对螺旋藻和发酵的链球菌（FS）的不同提取物的免疫刺激活性。结果表明，与未发酵的S. platensis相比，混合发酵增强了S. platensis的免疫调节活性，并具有更高的淋巴细胞增殖能力。（NFS）。值得注意的是，发酵的链球菌（L-PFS）的低分子量（<3 kDa）肽级分，特别是40μg/ ml，在促进淋巴细胞增殖和调节细胞因子（IL-2和IL-10）方面表现出最强的活性。 ）分泌物。同时，L-PFS增强了Th1细胞因子（IFN-γ）和Th2细胞因子（IL-4）的相对mRNA表达，同时抑制了Th1细胞因子（IL-2和TNF-α）和Th2细胞因子的相对mRNA表达（ IL-10）与伴刀豆球蛋白A处理的淋巴细胞进行比较。