Background

Some antibodies and autoreactive antibodies are associated with the severity of infectious diseases. The roles of humoral responses to lung inflammation in children with human adenovirus (HAdVs) pneumonia remain unknown.

Patients and methods

A retrospective study was done to compare plasma immunoglobulin E (IgE) levels between HAdVs pneumonia patients and healthy children by searching the electronic medical record system of Guangzhou Women and Children's Medical Center. Then, a prospective study was performed for children with HAdVs pneumonia who needed flexible bronchoscopy for examination and treatment purposes during July 2017 to July 2019. We examined the IgE and autoreactive IgE levels in plasma and bronchoalveolar lavage fluid (BALF) of these children to explore their role in HAdVs pneumonia.

Results

A significantly higher level of IgE was found in plasma from children hospitalized with HAdVs pneumonia compared with that from healthy children in the same age range. Furthermore, the levels of IgE, double-stranded DNA (dsDNA), and double-stranded DNA-specific immunoglobulin E (dsDNA-IgE) in BALF were increased compared to plasma in children with HAdVs pneumonia. The levels of IgE, dsDNA, and dsDNA-IgE in BALF were significantly higher in the severe group compared to the non-severe group. The ability of IgE in BALF to recognize dsDNA was verified by the ELISPOT test.

Conclusions

Our findings indicate that IgE and dsDNA-IgE in BALF may contribute to lung injury caused by HAdVs, especially in severe cases. Elevated dsDNA-IgE may serve as an indicator of severity in children with HAdVs pneumonia.

中文翻译：

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人腺病毒性肺炎患儿支气管肺泡灌洗液中的总和双链DNA特异性免疫球蛋白E。

背景

一些抗体和自身反应性抗体与传染病的严重程度有关。在人类腺病毒（HAdVs）肺炎患儿中，体液反应对肺部炎症的作用仍然未知。

患者和方法

通过检索广州市妇女儿童医疗中心的电子病历系统，进行了一项回顾性研究，以比较HAdVs肺炎患者和健康儿童的血浆免疫球蛋白E（IgE）水平。然后，对2017年7月至2019年7月需要进行柔性支气管镜检查和治疗的HAdVs肺炎患儿进行了一项前瞻性研究。我们检查了这些患儿血浆和支气管肺泡灌洗液（BALF）中的IgE和自身反应性IgE水平，以进行探索它们在HAdVs肺炎中的作用。

结果

与相同年龄段健康儿童的血浆相比，住院治疗的HAdVs肺炎儿童的血浆中的IgE水平明显更高。此外，与HAdVs肺炎患儿的血浆相比，BALF中的IgE，双链DNA（dsDNA）和双链DNA特异性免疫球蛋白E（dsDNA-IgE）水平升高。与非严重组相比，严重组BALF中的IgE，dsDNA和dsDNA-IgE水平显着更高。通过ELISPOT测试验证了BALF中IgE识别dsDNA的能力。

结论

我们的发现表明，BALF中的IgE和dsDNA-IgE可能导致HAdV引起的肺损伤，特别是在严重病例中。dsDNA-IgE升高可能是HAdVs肺炎患儿严重程度的指标。