当前位置: X-MOL 学术Brain Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The Vps52 subunit of the GARP and EARP complexes is a novel Arf6-interacting protein that negatively regulates neurite outgrowth of hippocampal neurons.
Brain Research ( IF 2.9 ) Pub Date : 2020-05-28 , DOI: 10.1016/j.brainres.2020.146905
Kanta Ibuchi 1 , Masahiro Fukaya 1 , Tetsuro Shinohara 1 , Yoshinobu Hara 1 , Tomoko Shiroshima 1 , Takeyuki Sugawara 1 , Hiroyuki Sakagami 1
Affiliation  

ADP ribosylation factor 6 (Arf6) is a small GTP-binding protein implicated in neuronal morphogenesis through endosomal trafficking and actin remodeling. In this study, we identified Vps52, a core subunit of the Golgi-associated retrograde protein (GARP) and endosome-associated recycling protein (EARP) complexes, as a novel Arf6-binding protein by yeast two-hybrid screening. Vps52 interacted specifically with GTP-bound Arf6 among the Arf family. Immunohistochemical analyses of hippocampal pyramidal cells revealed that fine punctate immunolabeling for Vps52 was distributed throughout neuronal compartments, most densely in the cell body and dendritic shafts, and was largely associated with trans-Golgi network and vesicular endomembranes. In cultured hippocampal neurons, knockdown of Vps52 increased total length of axons and dendrites; these phenotypes were completely restored by co-expression of shRNA-resistant full-length Vps52. However, co-expression of a Vps52 mutant lacking the ability to interact with Arf6 restored only the Vps52-knockdown phenotype of the dendritic length. The present findings suggest that Vps52 is a novel Arf6-interacting protein that regulates neurite outgrowth in hippocampal neurons.



中文翻译:

GARP 和 EARP 复合物的 Vps52 亚基是一种新型的 Arf6 相互作用蛋白,可负向调节海马神经元的轴突生长。

ADP 核糖基化因子 6 (Arf6) 是一种小的 GTP 结合蛋白,通过内体运输和肌动蛋白重塑参与神经元形态发生。在这项研究中,我们通过酵母双杂交筛选鉴定了 Vps52,它是高尔基体相关逆行蛋白 (GARP) 和内体相关循环蛋白 (EARP) 复合物的核心亚基,是一种新型的 Arf6 结合蛋白。Vps52 与 Arf 家族中 GTP 结合的 Arf6 特异性相互作用。海马锥体细胞的免疫组织化学分析表明,Vps52 的精细点状免疫标记分布在整个神经元隔室中,最密集的是细胞体和树突轴,并且主要与跨高尔基体网络和囊泡内膜相关。在培养的海马神经元中,Vps52 的敲低增加了轴突和树突的总长度;这些表型通过 shRNA 抗性全长 Vps52 的共表达完全恢复。然而,缺乏与 Arf6 相互作用能力的 Vps52 突变体的共表达仅恢复了树突长度的 Vps52 敲低表型。目前的研究结果表明,Vps52 是一种新型的 Arf6 相互作用蛋白,可调节海马神经元中的神经突生长。

更新日期:2020-05-28
down
wechat
bug