The pathogenesis of primary focal hyperhidrosis (PFH) is still not clear. PFH is thought to be a genetic disease. Whether activin A receptor type 1 (ACVR1) is involved in the pathogenesis of PFH is unknown. In this study, the expression of ACVR1 in sweat glands of patients with PAH was detected by western blot and immunofluorescence. The primary sweat gland cells obtained from primary axillary hyperhidrosis (PAH) patients were transfected with acvr1 vector. Cell proliferation, apoptosis and cell cycling of gland cells were measured after transfection with acvr1 vector. The mRNA and protein expression of aquaporin 5 (AQP5) and Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) were detected. Our data showed that ACVR1 expression in axillary sweat gland tissue of PAH patients was significantly higher than that of normal control group. The function of ACVR1 was further investigated in the gland cells obtained from PAH patients. Compared with NC group, ACVR1 overexpression significantly promoted the proliferation of sweat gland cells and inhibited the apoptosis of sweat gland cells. Meanwhile, ACVR1 overexpression significantly reduced the percentage of cells in G0/G1 and G2/M phases, and increased the percentage of cells in S phase. In addition, ACVR1 overexpression significantly promoted the expression of AQP5 and NKCC1 at both mRNA and protein levels. Together, ACVR1 expression is related to PFH and ACVR1 overexpression can promote the proliferation of sweat gland cells and inhibit apoptosis by promoting the expression of AQP5 and NKCC1.

原发灶性多汗症（PFH）的发病机制仍不清楚。PFH被认为是一种遗传性疾病。激活素A 1型受体（ACVR1）是否参与PFH的发病机理尚不清楚。在这项研究中，通过蛋白质印迹和免疫荧光检测ACVR1在PAH患者汗腺中的表达。用acvr1载体转染从原发性腋窝多汗症（PAH）患者获得的原发汗腺细胞。acvr1转染后检测腺细胞的细胞增殖，凋亡和细胞周期向量。检测水通道蛋白5（AQP5）和Na：K：2Cl共转运蛋白1（NKCC1 / SLC12A2）的mRNA和蛋白表达。我们的数据显示，PAH患者腋窝汗腺组织中的ACVR1表达明显高于正常对照组。在从PAH患者获得的腺细胞中进一步研究了ACVR1的功能。与NC组相比，ACVR1过表达显着促进汗腺细胞增殖，抑制汗腺细胞凋亡。同时，ACVR1过表达显着降低了G0 / G1和G2 / M期的细胞百分比，并增加了S期的细胞百分比。此外，ACVR1的过表达在mRNA和蛋白质水平上均显着促进AQP5和NKCC1的表达。一起，