Connexin 30 (Cx30), a tumour-suppressive gap junctional protein, impacts on insulin-like growth factor receptor 1-mediated progression and stemness of glioma. Of late, metabolic reprogramming, a recently adjudged hall mark of malignancy, could reasonably associated with the changes in gap junctional communication in glioma. This newly recognized hallmark of reprogramming of metabolism to maintain the rapid proliferation necessitates further probing to establish the stronger hall marks. Hence, the current study attempted to link the association between the expression of Cx30 with glucose uptake and glucose metabolism in glioma. We have transfected Cx30 in C6 glioma cells, characterized by a low level of intercellular communication and developed xenografts to study the status of glucose transporters (GLUTs), hexokinase 2 and Pyruvate dehydrogenase kinase 1 (PDK 1) along with human glioma tissues by RT-PCR and immunoblotting. The results showed a significant increase in the levels of GLUTs, hexokinase 2 and PDK 1 in C6-implanted rat xenografts and high grades compared to their respective controls, whereas Cx30-transfected C6-implanted rat xenograft and low grades show no significant change compared to that of controls supporting the association between Gap junctional communications and glucose metabolism. We strongly speculate the impact of Cx30 over the glucose metabolism that might provide therapeutic prospects and challenges for anti-glycolytic cancer therapy.

连接蛋白30（Cx30），一种抑制肿瘤的间隙连接蛋白，影响胰岛素样生长因子受体1介导的神经胶质瘤的进展和干性。最近，新近确定的恶性肿瘤新陈代谢重新编程可合理地与神经胶质瘤的间隙连接通讯改变有关。这种新近认识到的重新编程新陈代谢以维持快速增殖的标志需要进一步探索以建立更强的标志。因此，当前的研究试图将胶质瘤中Cx30表达与葡萄糖摄取和葡萄糖代谢之间的联系联系起来。我们已经转染了C6胶质瘤细胞中的Cx30，其特征是细胞间通讯水平较低，并开发了异种移植物以研究葡萄糖转运蛋白（GLUT）的状态，己糖激酶2和丙酮酸脱氢酶激酶1（PDK 1）以及人类神经胶质瘤组织通过RT-PCR和免疫印迹分析。结果显示，与各自的对照相比，C6植入的大鼠异种移植物中GLUTs，己糖激酶2和PDK 1的水平显着提高，而与对照相比，Cx30转染的C6移植的大鼠异种移植物和低等级中的GLUTs，己糖激酶2和PDK 1的水平没有显着变化。支持间隙连接通讯和葡萄糖代谢之间的关联的控件。我们强烈推测Cx30对葡萄糖代谢的影响，这可能为抗糖酵解性癌症治疗提供治疗前景和挑战。C6植入的大鼠异种移植物中的己糖激酶2和PDK 1与它们各自的对照相比为高等级，而Cx30转染的C6植入的大鼠异种移植物和低等级与支持间隙连接通讯和葡萄糖之间关联的对照相比，没有显示出显着变化。代谢。我们强烈推测Cx30对葡萄糖代谢的影响，这可能为抗糖酵解性癌症治疗提供治疗前景和挑战。C6植入的大鼠异种移植物中的己糖激酶2和PDK 1与它们各自的对照相比等级较高，而Cx30转染的C6植入的大鼠异种移植物和低等级与支持间隙连接通讯和葡萄糖之间关联的对照相比，没有显示出显着变化代谢。我们强烈推测Cx30对葡萄糖代谢的影响，这可能为抗糖酵解性癌症治疗提供治疗前景和挑战。