We designed two novel primer sets based on the sequence of the hrpZ gene to detect Pseudomonas syringae pv. syringae, the causal agent of bacterial black node in barley and wheat. A conventional PCR using these primers amplified only P. syringae group III strains. The result of a nested PCR with the novel primers showed that P. syringae pv. syringae was detectible even when only one culturable cell was used as the PCR template. This highly sensitive PCR detected P. syringae in diseased parts of wheat and barley plants and in asymptomatic plants.

中文翻译：

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丁香假单胞菌PV的PCR检测。新设计的引物对大丁香和小麦中细菌黑节病的病原体丁香

我们基于hrpZ基因的序列设计了两个新颖的引物组，以检测丁香假单胞菌pv。丁香科，大麦和小麦中细菌性黑结的病原体。使用这些引物扩增只有A常规PCR P。丁香三类菌株。用新的引物巢式PCR的结果表明，P。丁香科植物 即使仅将一个可培养细胞用作PCR模板，丁香也可检出。这一高度敏感PCR检测P。小麦和大麦植物患病部位以及无症状植物中的丁香科。