This study investigated the survivals of two pathogens (Escherichia coli O157:H7 and Staphylococcus aureus) in different adhered forms on glass fiber filters (GFFs) at 43 and 68% relative humidity (RH). Efficacies of chemical sanitizers at reducing pathogenic biofilms on GFFs were also evaluated. Inoculated GFFs were incubated at 28 °C in TSB (type I), on TSA (type II), or on TSA under 100% RH (type III) to produce biofilms. When GFFs were incubated at 43 or 68% RH for 7 days, type III biofilms were less than 2 log₁₀ CFU/filter reduction whereas type I and type II biofilms were 4–6 log₁₀ CFU/filter reduction. Additionally, type III biofilms were highly resistant to sanitizing treatment compared than other biofilms (type I and II). Therefore, the method to produce biofilms used in this study could be used to produce highly resistance pathogenic biofilms in the laboratory for related experiments.

这项研究调查了两种病原体（大肠杆菌O157：H7和金黄色葡萄球菌）在玻璃纤维过滤器（GFF）上在43％和68％相对湿度（RH）下的不同粘附形式的存活率。还评估了化学消毒剂在减少GFF上的致病性生物膜方面的功效。将已接种的GFF在28°C下于TSB（I型），TSA（II型）或TSA于100％RH（III型）下孵育，以产生生物膜。当GFF在43或68％相对湿度下孵育7天时，III型生物膜小于2 log ₁₀ CFU /过滤器减少，而I型和II型生物膜则为4-6 log ₁₀CFU /过滤器减少。此外，与其他生物膜（I和II型）相比，III型生物膜对消毒处理具有更高的抵抗力。因此，本研究中使用的生产生物膜的方法可用于实验室中用于相关实验的高抗病原性生物膜的生产。