Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of compounds. It is most promising to develop simple and effective biosensors for the use in situations where traditional methods are not available due to their complexity and laboriousness. In this project, a novel smart deoxyribozyme-based fluorescent sensor for the detection of androgen receptor mRNA was developed. It consists of several functional modules including two deoxyribozymes 10–23, an RNA-dependent split malachite green aptamer, and an oligonucleotide platform. Deoxyribozymes specifically release a 27-nucleotide RNA fragment that is readily available for the interaction with the aptamer module. This solves a problem of secondary structure in hybridization with the target sequence of full-length mRNA. It was shown that within 24 hours the proposed sensor specifically recognized both a synthetic 60-nucleotide RNA fragment (LOD is 1.4 nM of RNA fragment at 37 °C) and a full-sized mRNA molecule of the androgen receptor. The constructed sensor is easy to use, has high efficiency and selectivity for the RNA target, and can be reconstructed for the detection of various nucleic acid sequences due to its modular structure. Thus, similar biosensors may be useful for the differential diagnosis.

中文翻译：

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基于智能脱氧核酶的荧光传感器，用于体外检测雄激素受体mRNA。

如今，各种生物传感器已广泛应用于不同领域，包括生物医学诊断和自检。基于核酸的生物传感器通常用于检测另一种核酸，蛋白质，离子和几种其他类型的化合物。开发简单有效的生物传感器以用于传统方法由于其复杂性和费力而无法使用的情况下，是最有希望的。在这个项目中，开发了一种新型的基于智能脱氧核酶的荧光传感器，用于检测雄激素受体的mRNA。它由几个功能模块组成，包括两个脱氧核酶10-23，一个RNA依赖的孔雀石绿适体和一个寡核苷酸平台。脱氧核酶特异性释放27个核苷酸的RNA片段，该片段易于与适体模块相互作用。这解决了与全长mRNA的靶序列杂交中的二级结构的问题。结果表明，拟议的传感器在24小时内能特异性识别合成的60个核苷酸的RNA片段（在37°C下LOD为1.4 nM RNA片段）和雄激素受体的全尺寸mRNA分子。所构建的传感器易于使用，对RNA靶标具有高效率和选择性，并且由于其模块化结构而可以被重构用于检测各种核酸序列。因此，类似的生物传感器对于鉴别诊断可能是有用的。在37°C下4 nM的RNA片段）和雄激素受体的全尺寸mRNA分子。所构建的传感器易于使用，对RNA靶标具有高效率和选择性，并且由于其模块化结构而可以被重构用于检测各种核酸序列。因此，类似的生物传感器对于鉴别诊断可能是有用的。在37°C下4 nM的RNA片段）和雄激素受体的全尺寸mRNA分子。所构建的传感器易于使用，对RNA靶标具有高效率和选择性，并且由于其模块化结构而可以被重构用于检测各种核酸序列。因此，类似的生物传感器对于鉴别诊断可能是有用的。