Protein nanoparticles have attracted widespread attention in the area of biocatalysis, drug delivery, and vaccine preparation during the past decade. Among various protein nanostructures, protein nanocages have proved to be a multifunctional platform for tumor diagnosis and drug delivery due to easy interior encapsulation and outer surface display. In this report, a protein‐only nanoparticle (HSP‐KLAK) is developed based on small heat shock protein (HSP) nanocage and proapoptotic peptide (KLAK) for enhanced antitumor activity. The HSP‐KLAK nanoparticles show efficient cellular uptake and good lysosomal escape in melanoma cells. Moreover, HSP‐KLAK nanoparticles show significantly enhanced cytotoxicity toward B16F10 melanoma cells compared with free proapoptotic peptide. This study demonstrates that protein nanocage represents an efficient nanocarrier for delivering anticancer peptide into tumors.

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通过HSP Nanocage传递促凋亡肽用于癌症治疗

在过去的十年中，蛋白质纳米颗粒在生物催化，药物输送和疫苗制备领域引起了广泛的关注。在各种蛋白质纳米结构中，由于易于内部封装和外表面展示，蛋白质纳米笼已被证明是用于肿瘤诊断和药物递送的多功能平台。在本报告中，基于小型热休克蛋白（HSP）纳米笼和促凋亡肽（KLAK）的纯蛋白质纳米颗粒（HSP-KLAK）被开发出来，以增强抗肿瘤活性。HSP-KLAK纳米颗粒在黑素瘤细胞中显示出有效的细胞摄取和良好的溶酶体逃逸。此外，与游离促凋亡肽相比，HSP‐KLAK纳米颗粒对B16F10黑色素瘤细胞显示出显着增强的细胞毒性。