Rainbow trout ( Oncorhynchus mykiss ) identification in processed fish products using loop‐mediated isothermal amplification ( LAMP ) and PCR assays,Journal of the Science of Food and Agriculture

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Rainbow trout ( Oncorhynchus mykiss ) identification in processed fish products using loop‐mediated isothermal amplification ( LAMP ) and PCR assays
Journal of the Science of Food and Agriculture ( IF 4.1 ) Pub Date : 2020-07-03 , DOI: 10.1002/jsfa.10526
Xiong Xiong ₁ , Manhong Huang ₁ , Wenjie Xu ₁ , Yi Li ₁ , Min Cao ₁ , Xiaohui Xiong ₁

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BACKGROUND Financial loss and health risk caused by the substitution of rainbow trout for other salmonid species, have become a common issue around the world. The situation could be further exacerbated in China by the 'abused' common name of San Wen Yu (the corresponding Chinese ideogram ) for salmonids, considering the absence of a standardized naming system for seafood species. To prevent such episodes, the present study aimed to develop novel loop mediated isothermal amplification (LAMP) and PCR assays targeting the mitochondrial cytochrome b (cytb) gene for rapid identification of rainbow trout in processed fish products. RESULTS Rainbow trout-specific primers (LAMP and PCR) were designed, and the specificity against 23 different fish species was confirmed. The minimum amount of the detectable DNA for LAMP assay reached 500 pg, up to 10-fold less than PCR assay. In addition to the agarose gel electrophoresis, naked-eye inspection of the LAMP-positive samples using SYBR Green I under daylight or ultraviolet was also validated. Finally, commercial San Wen Yu products made from rainbow trout could be accurately identified, using the newly developed LAMP and PCR assays, further cross-confirmed by the mini DNA barcoding and Neighbour-joining dendrogram. CONCLUSION The established LAMP and PCR assays in the present study allow a fast and accurate identification of rainbow trout in processed fish products. This article is protected by copyright. All rights reserved.

更新日期：2020-07-03

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