This research work examined and likened effect of eugenol a natural phenolic compound with butylated hydroxylanisole (BHA) and butylated hydroxyl toluene (BHT) synthetic phenolic compounds with key biomolecules [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and monoamine oxidase (MAO)] relevant to Alzheimer's diseases (AD) in vitro. Ten millimolar each sample was prepared in a mixture of ethanol and water (1:1 v/v), and the interactions with AChE, BChE, and MAO were evaluated. Still, ferric reducing antioxidant property, ABTS radicals scavenging ability and lipid peroxidation were carried out. The results revealed eugenol, BHT, and BHA inhibited AChE, BChE, and MAO activities dose‐dependently. Though, eugenol had greater inhibitory effect against AChE and BChE activities. Also, eugenol demonstrated higher antioxidant potential compared to BHT and BHA. The potent enzymatic inhibitory and antioxidant effects of eugenol indicate eugenol could be promising as an alternative food additive and neuromodulator in AD management.

中文翻译：

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丁香酚，丁基化羟基茴香醚和丁基化羟基甲苯与与阿尔茨海默氏病有关的关键生物分子的抗氧化性能

这项研究工作研究了丁香酚与天然丁香酚与丁基化羟基茴香醚（BHA）和丁基化羟基甲苯（BHT）合成酚类化合物与关键生物分子[乙酰胆碱酯酶（AChE），丁酰胆碱酯酶（BChE）和单胺氧化酶（MAO）]的作用并进行了比较。与体外阿尔茨海默氏病（AD）有关。在乙醇和水的混合物（1：1 v / v）中制备每个样品10毫摩尔，并评估与AChE，BChE和MAO的相互作用。仍进行了还原铁的抗氧化性能，ABTS自由基清除能力和脂质过氧化反应。结果表明丁子香酚，BHT和BHA剂量依赖性地抑制AChE，BChE和MAO活性。虽然，丁子香酚对AChE和BChE活性具有更大的抑制作用。还，丁子香酚比BHT和BHA具有更高的抗氧化潜力。丁子香酚的强大的酶促抑制和抗氧化作用表明，丁子香酚可以作为AD管理中的替代食品添加剂和神经调节剂。