Dual‐pump electrospinning of antibacterial N‐decyl‐N, N‐dimethyl‐1‐decanaminium‐chloride (DDAC)‐loaded polycaprolactone (PCL) nanofibers, and chitosan (CS)/polyethylene‐oxide (PEO)‐based wound dressings with hydrophilic and hydrophobic properties to eliminate and absorb pathogenic bacteria from wound surface besides antibacterial action and to support wound healing and accelerate its process. Physicochemical properties of the prepared nanofibrous mat as well as antibacterial, cytotoxicity, and cell compatibility were studied. The full‐thickness excisional wound healing properties up to 3 weeks using hematoxylin and eosin and Masson‐trichrome staining were investigated. Addition of DDAC to CS/PEO‐PCL mats decreased the diameter of the nanofibers, which is a crucial property for wound healing as large surface area per volume ratio of nanofibers, in addition to proper cell adhesion, increases loading of DDAC in mats and leads to increased cell viability and eliminating Gram‐positive bacteria at in vitro studies. In vivo studies showed DDAC‐loaded CS/PEO‐PCL mats increased epithelialization and angiogenesis and decreased the inflammation according to histological results. We demonstrated that hydrophobic PCL/DDAC mats, besides antibacterial properties of DDAC, absorbed and eliminated the hydrophobic pathological microorganisms, whereas the hydrophilic nanofibers consisted of CS/PEO, increased the cell adhesion and proliferation due to positive charge of CS. Finally, we were able to increase the wound healing quality by using multifunctional wound dressing. CS/PEO‐PCL containing 8 wt % of DDAC nanofibrous mats is promising as a wound dressing for wound management due to the favorable interactions between the pathogenic bacteria and PCL/CS‐based wound dressing.

中文翻译：

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负载 N-癸基-N、N-二甲基-1-癸胺氯化物的混合电纺聚己内酯/壳聚糖基伤口敷料的物理和生物学特性：一项体外和体内研究。

抗菌N-癸基-N、N-二甲基-1-癸胺-氯化物（DDAC）负载的聚己内酯（PCL）纳米纤维和具有亲水性的壳聚糖（CS）/聚环氧乙烷（PEO）基伤口敷料的双泵静电纺丝和疏水特性，除了抗菌作用外，还可以消除和吸收伤口表面的病原菌，并支持伤口愈合并加速其进程。研究了制备的纳米纤维垫的理化性质以及抗菌、细胞毒性和细胞相容性。使用苏木精和伊红以及马森三色染色研究了长达 3 周的全层切除伤口愈合特性。将 DDAC 添加到 CS/PEO-PCL 垫中可减小纳米纤维的直径，这是伤口愈合的关键特性，因为纳米纤维的单位体积比表面积大，除了适当的细胞粘附外，增加垫中 DDAC 的负载并导致细胞活力增加并在体外研究中消除革兰氏阳性菌。体内研究表明，根据组织学结果，载有 DDAC 的 CS/PEO-PCL 垫增加了上皮化和血管生成并减少了炎症。我们证明了疏水性 PCL/DDAC 垫除了具有 DDAC 的抗菌特性外，还可以吸收和消除疏水性病理微生物，而由 CS/PEO 组成的亲水性纳米纤维由于 CS 带正电荷而增加了细胞粘附和增殖。最后，我们能够通过使用多功能伤口敷料来提高伤口愈合质量。