There is a growing clinical demand in the wound care market to treat chronic wounds such as diabetic foot ulcers. Advanced cell and tissue‐based products (CTPs) are often used to address challenging chronic wounds where healing has stalled. These products contain active biologics such as growth factors and cytokines as well as structural components that support and stimulate cell growth and assist in tissue regeneration. This study addresses the in vitro biologic effects of a clinically available dehydrated amniotic membrane allograft (DAMA). The broad mechanism of action results from DAMA's biologic composition that leads to stimulation of cell migration cell proliferation, and reduction of pro‐inflammatory cytokines. Results show that DAMA possesses growth factors and cytokines such as EGF, FGF, PDGFs, VEGF, TGF‐β, IL‐8, and TIMPs 1 and 2. Furthermore, in vitro experiments demonstrate that DAMA stimulates cell proliferation, cell migration, secretion of collagen type I, and the reduction of pro‐inflammatory cytokines IL‐1β, IL‐6, and TNF‐α. This study findings are consistent with the clinical benefits previously published for DAMA and other CTPs in chronic wounds suggesting that the introduction of DAMA to non‐healing, complex wounds helps to improve the wound milieu by providing essential structural components, cytokines, and growth factors to create an appropriate environment for wound healing.

中文翻译：

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脱水羊膜同种异体移植物的生物学特性：作用机制和对伤口护理的影响。

伤口护理市场对治疗糖尿病足溃疡等慢性伤口的临床需求不断增长。先进的细胞和组织产品 (CTP) 通常用于解决愈合停滞的具有挑战性的慢性伤口。这些产品含有活性生物制剂，如生长因子和细胞因子，以及支持和刺激细胞生长并协助组织再生的结构成分。该研究解决了体外生物学效应临床上可用的脱水羊膜同种异体移植物 (DAMA)。广泛的作用机制源于 DAMA 的生物成分，可刺激细胞迁移、细胞增殖和减少促炎细胞因子。结果表明 DAMA 具有生长因子和细胞因子，如 EGF、FGF、PDGFs、VEGF、TGF-β、IL-8 和 TIMPs 1 和 2。此外，体外实验表明 DAMA 刺激细胞增殖、细胞迁移、分泌I 型胶原蛋白，以及促炎细胞因子 IL-1β、IL-6 和 TNF-α 的减少。该研究结果与之前发表的 DAMA 和其他 CTP 在慢性伤口中的临床益处一致，表明将 DAMA 引入到不愈合的伤口中，