Metformin (MET) is an antidiabetic drug most commonly used in treatment of diabetes mellitus type 2 (T2D). Adsorptive stripping voltammetric method using carbon black – hydrated ruthenium dioxide – Nafion modified glassy carbon electrode (CB‐RuO₂‐Nafion GC electrode) have been developed for metformin determination in pharmaceutical formulations. By using ruthenium dioxide, electrode's lifespan was extended to at least 3 weeks (change of metrological parameters estimated as 3–4 %) what is an excellent result concerning other solutions previously described in the literature. Moreover the fabrication of the sensor is simple and fast. Deposition step was carried out at the potential 0 mV for 15 s. The best results were obtained in 0.05 M acetate buffer (pH 4.6). Important aspect was fixed MET : Cu(II) ratio equal to 1 : 8, otherwise linear dependence between register current and MET concentration could not be obtained. In addition, a significant improvement in the parameters of the calibration curve was obtained. Limit of detection was equal to 0.7 μM. Developed method was successfully applied in analysis of 2 pharmaceuticals products and in wastewater and river water. Accuracy of the method was estimated using recoveries, which were in the range 101–110 %. In order to adapt developed system into hydrodynamic conditions, amperometry in hydrodynamic transport conditions and flow injection analysis (FIA) measurements have been conducted. Conducted FIA measurements prove that developed method has potential for application in automized flow systems without frequent calibration.

中文翻译：

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水合二氧化钌-碳黑-Nafion修饰玻碳电极测定二甲双胍的新型电化学传感器

二甲双胍（MET）是一种抗糖尿病药物，最常用于治疗2型糖尿病（T2D）。炭黑-水合二氧化钌-Nafion修饰玻碳电极（CB-RuO _2）的吸附溶出伏安法‐Nafion GC电极）已开发用于测定药物制剂中的二甲双胍。通过使用二氧化钌，电极的寿命至少延长了3周（计量参数的变化估计为3-4％），这与先前文献中描述的其他解决方案相比是极好的结果。此外，传感器的制造简单且快速。沉积步骤在0 mV的电位下进行15 s。在0.05 M乙酸盐缓冲液（pH 4.6）中获得了最佳结果。重要方面是固定的MET：Cu（II）比等于1：8，否则无法获得寄存器电流和MET浓度之间的线性关系。另外，在校准曲线的参数上获得了显着的改善。检测限等于0.7μM。所开发的方法已成功用于两种药物产品以及废水和河水中的分析。使用回收率估算方法的准确性，回收率在101-110％的范围内。为了使已开发的系统适应水动力条件，已进行了水动力运输条件下的安培计和流动注射分析（FIA）测量。进行的FIA测量证明，开发的方法具有无需频繁校准即可应用于自动流量系统的潜力。在流体动力运输条件下进行安培法和流动注射分析（FIA）测量。进行的FIA测量证明，开发的方法具有无需频繁校准即可应用于自动流量系统的潜力。在流体动力运输条件下进行安培法和流动注射分析（FIA）测量。进行的FIA测量证明，开发的方法无需频繁进行校准即可在自动化流量系统中应用。