Organic fluorophores are essential in microscopic imaging of biological systems. New fluorophores with distinct features are in demand for bioimaging. Herein we report new bent benzocoumarin dyes that are fluorescent both in solution and in the solid state. The latter property enables us to form fluorescent nanoparticles with a mean size of 120–157 nm or larger depending on conditions. Some of the new dyes provide bright fluorescent images in cell, with formation of particulate spots that persist even after several washout procedures, and, furthermore, in tissue under two‐photon excitation at 900 nm. Such dyes have potential for the development of fluorescent probes for enhanced cellular fluorescence.

中文翻译：

---

溶液和固态中发出荧光的弯曲苯并香豆素染料及其在生物成像中的应用

有机荧光团在生物系统的显微成像中必不可少。具有生物特征的新型荧光团是需要的。本文中，我们报告了在溶液和固态下都发荧光的新型弯曲苯并香豆素染料。后者的特性使我们能够根据条件形成平均尺寸为120–157 nm或更大的荧光纳米粒子。一些新染料可在细胞内提供明亮的荧光图像，甚至在经过数次冲洗程序后，甚至在900 nm的双光子激发下的组织中，仍会形成颗粒状斑点。这种染料具有开发用于增强细胞荧光的荧光探针的潜力。