For successful implantation, endometrial receptivity must be established. The high expression of CDC20 in many kinds of malignant tumours has been reported, and it is related to the occurrence and development of tumours. According to these functions, we think that CDC20 may also play important roles in the process of embryo implantation. To prove our hypothesis, we observed the distribution and expression of CDC20 in mouse and human early pregnancy. The effect of E2 and/or P4 on the expression of CDC20 in human endometrial cells was detected by Western blot. To further explore whether CDC20 is an important factor in adhesion and proliferation. The results showed that the expression of CDC20 in the uterus and menstrual cycle of early pregnant mice was spatiotemporal. E2 can promote the expression of CDC20. On the contrary, P4 and E2 + P4 inhibited the expression of CDC20. We also detected the proliferation and adhesion of human endometrial cells. We found that the inhibition of CDC20 with its inhibitor Apcin could reduce the adhesion rate and proliferation ability to RL95‐2 and HEC‐1A cells, respectively. Inhibiting CDC20 by Apcin could interfere the embryo implantation of mouse. It is suggested that CDC20 may play an important role in the process of embryo implantation.

中文翻译：

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CDC20抑制剂Apcin可在体内和体外抑制胚胎植入。

为了成功植入，必须建立子宫内膜的接受能力。已经报道了CDC20在多种恶性肿瘤中的高表达，这与肿瘤的发生和发展有关。根据这些功能，我们认为CDC20在胚胎植入过程中也可能起重要作用。为了证明我们的假设，我们观察了CDC20在小鼠和人类早期妊娠中的分布和表达。通过Western印迹检测E2和/或P4对人子宫内膜细胞CDC20表达的影响。进一步探讨CDC20是否是粘附和增殖的重要因素。结果表明，CDC20在早孕小鼠子宫和月经周期中的表达呈时空分布。E2可以促进CDC20的表达。反之，P4和E2 + P4抑制CDC20的表达。我们还检测了人类子宫内膜细胞的增殖和粘附。我们发现用其抑制剂Apcin抑制CDC20可以分别降低对RL95-2和HEC-1A细胞的粘附率和增殖能力。Apcin抑制CDC20可能会干扰小鼠的胚胎植入。提示CDC20可能在胚胎植入过程中起重要作用。