The aim of this study was to develop an effective method for extracting anthocyanins from blueberry Vaccinium spp. (ABVS) using freeze-ultrasonic thawing technology (FUTE). Various parameters including freezing time, ultrasonic time, ultrasonic temperature and liquid–solid ratio were optimized by a single-factor design and multiple response surface methodology. The amounts of extracted anthocyanin and cyanidin-3-O-glucoside were measured by UV and HPLC respectively. The maximum yield of anthocyanins was achieved by freezing the samples for 5.43 min in liquid nitrogen at the liquid–solid ratio of 24.07:1 mL/g, followed by ultrasonic thawing at 41.64 °C for 23.56 min. The yield and antioxidant effects of ABVS extracted using FUTE, ultrasound-assisted extraction (UAE) and freeze-thawing extraction (FTE) were compared in order to determine the overall efficacy of FUTE. In addition to the higher content, FUTE extracted ABVS showed greater ability to scavenge DPPH·, ABTS⁺ and superoxide anions, and inhibit lipid peroxidation compared to the ABVS extracted by UAE or FTE. The reducing power of the FUTE-derived ABVS was intermediate between that of the UAE and FTE samples. Taken together, FUTE can rapidly and effectively extract ABVS and retain its antioxidant capacity.

这项研究的目的是开发一种从蓝莓越桔中提取花青素的有效方法。spp。（ABVS）使用冻融解冻技术（FUTE）。通过单因素设计和多重响应面方法优化了各种参数，包括冷冻时间，超声时间，超声温度和液固比。分别通过UV和HPLC测量提取的花色苷和花青素-3-O-葡萄糖苷的量。通过在液氮中以24.07：1 mL / g的液固比将样品在液氮中冷冻5.43分钟，然后在41.64°C超声解冻23.56分钟，来获得最大的花色苷产量。比较了用FUTE提取的ABVS，超声辅助提取（UAE）和冻融提取（FTE）的产量和抗氧化作用，以确定FUTE的整体功效。除了更高的内容，⁺和超氧阴离子，与通过阿联酋或FTE提取的ABVS相比，抑制脂质过氧化。源自FUTE的ABVS的还原能力介于阿联酋和FTE样品之间。总之，FUTE可以快速有效地提取ABVS，并保持其抗氧化能力。