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LncRNA GAS5 participates in the regulation of dexamethasone on androgen receptor -negative and -positive prostate cancer cell proliferation.
Molecular and Cellular Probes ( IF 3.3 ) Pub Date : 2020-05-26 , DOI: 10.1016/j.mcp.2020.101607
Jieping Hu 1 , Jun Deng 1 , Runfu Cao 1 , Shida Xiong 1 , Ju Guo 1
Affiliation  

Background

Androgen receptor (AR) and long non-coding RNAs (lncRNA) play important roles in the initiation and progression of prostate cancer (PCa). The present study was designed to investigate whether lncRNA growth arrest-specific 5 (GAS5) is involved in the regulation of dexamethasone on the proliferation of AR+ PCa and AR PCa cell lines.

Methods

Cell proliferation and cell cycle distribution were assessed using MTT assay and flow cytometry, respectively. GAS5 expression was examined by quantitative real-time PCR. AR protein level was examined by Western blot. RNA immunoprecipitation and RNA pull-down were performed to analyze the binding of GAS5 to AR.

Results

In AR PCa cell line PC3, dexamethasone upregulated GAS5 expression, induced cell cycle arrest in the G0/G1 phase and inhibited cell proliferation, which were enhanced by GAS5 overexpression and attenuated by GAS5 silencing. However, in AR+ PCa cell line 22Rv1, dexamethasone had no obvious effects on GAS5 expression, cell cycle distribution and cell proliferation. AR was localized in the cytoplasm and bound to GAS5, counteracting the proliferation-inhibitory effect of GAS5.

Conclusion

Taken together, GAS5 participates in the regulation of dexamethasone on the proliferation of AR+ PCa and AR PCa cell lines.



中文翻译:

LncRNA GAS5参与地塞米松对雄激素受体阴性和阳性前列腺癌细胞增殖的调节。

背景

雄激素受体(AR)和长非编码RNA(lncRNA)在前列腺癌(PCa)的发生和发展中起重要作用。本研究旨在研究lncRNA生长停滞特异性5(GAS5)是否参与地塞米松对AR + PCa和AR - PCa细胞系增殖的调控。

方法

分别使用MTT测定法和流式细胞仪评估细胞增殖和细胞周期分布。通过定量实时PCR检查GAS5表达。通过蛋白质印迹检查AR蛋白水平。进行RNA免疫沉淀和RNA下拉分析GAS5与AR的结合。

结果

在AR - PCa细胞系PC3中,地塞米松上调GAS5表达,诱导细胞周期阻滞在G0 / G1期,并抑制细胞增殖,这被GAS5过表达增强并被GAS5沉默减弱。然而,在AR + PCa细胞系22Rv1中,地塞米松对GAS5表达,细胞周期分布和细胞增殖没有明显影响。AR位于细胞质中并与GAS5结合,抵消了GAS5的增殖抑制作用。

结论

总之,GAS5参与地塞米松对AR + PCa和AR - PCa细胞系增殖的调控。

更新日期:2020-06-23
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