The aim of this research was to evaluate the effects of the temperament of bovine females Bos taurus indicus submitted to a timed artificial insemination (TAI) protocol on the concentrations of cortisol, diameter of the largest follicle, pregnancy by TAI on Days 30 (P/TAI 30) and 60 (P/TAI 60) after TAI. Nellore cows (n = 2702) of 45 ± 7 days post-partum were submitted to the same TAI protocol. On D -10 devices containing 1 g of progesterone were inserted into the vagina and 2 mg of estradiol benzoate were applied intramuscularly. On D -2, the devices were removed and 530 µg of sodium D-cloprostenol, 300 IU of equine chorionic gonadotropin, and 1 mg of Estradiol Cypionate were applied. Forty-eight hours after the final injection, the cows were submitted to TAI (D 0). The temperament of the cows was rated on a numerical scale from 3 to 12 using the composite reactivity score (RS) on D -10. The RS included the evaluation of the movement and breathing while the animal was in the cattle chute and the exit velocity. Cows were classified as Calm (RS ≤ 4; n = 830; Calm group), Reactive (RS = 5 to 8; n = 1313; Reactive group) and Very Reactive (RS ≥ 9; n = 559; Very Reactive group) and 5% of the cows of each group were submitted to blood collection for the subsequent plasmatic cortisol concentration measurement by ELISA. It was observed that Calm group had lower concentration of cortisol than did the Reactive and Very Reactive groups (35.07 ± 1.78, 50.58 ± 2.76, and 54.45 ± 3.39 nmol/L, respectively). The Calm group had diameter of the largest follicle on the day of TAI compared to Reactive and Very Reactive groups (14.4 ± 0.2, 13.6 ± 0.4, and 13.2 ± 0.2 mm, respectively). The Calm and Reactive groups had higher P/TAI compared to the Very Reactive Group on Day 30 (56.4%, 57.0%, 51.0%, respectively) and on Day 60 (50.0%, 47.3%, 42.7%, respectively). In conclusion, temperament influences the reproductive efficiency of bovine females Bos taurus indicus submitted to TAI protocols.

这项研究的目的是评估牛女性气质的影响，普通牛蜓在TAI后第30天（P / TAI 30）和60（P / TAI 60）接受了定时人工授精（TAI）方案的皮质醇浓度，最大卵泡直径，TAI妊娠。产后45±7天的内罗尔牛（n = 2702）接受相同的TAI方案。在D -10上，将含有1 g孕酮的器械插入阴道，并肌肉注射2 mg雌二醇苯甲酸酯。在D -2上，移去装置，并施加530 µg D-氯前列醇钠，300 IU马绒毛膜促性腺激素和1 mg雌二醇环戊酸酯。最后一次注射后四十八小时，将母牛接受TAI（D 0）。使用D -10上的复合反应分数（RS），以3到12的数字范围对母牛的气质进行评级。RS包括对动物在牛斜道中的运动和呼吸以及出口速度的评估。母牛分为：平静（RS≤4； n = 830；平静组），反应性（RS = 5至8； n = 1313；反应性组）和反应性强（RS≥9； n = 559；反应性强）组和每组5％的母牛接受血液收集，以用于随后通过ELISA测量血浆皮质醇浓度。观察到，平静组的皮质醇浓度低于反应性和非常反应性组（35.07±1.78，50.58 高度反应性组）和每组5％的母牛接受血液采集，以用于随后通过ELISA测定血浆皮质醇浓度。观察到，平静组的皮质醇浓度低于反应性和高度反应性组（35.07±1.78，50.58 高度反应性组）和每组5％的母牛接受血液采集，以用于随后通过ELISA测定血浆皮质醇浓度。观察到，平静组的皮质醇浓度低于反应性和高度反应性组（35.07±1.78，50.58分别为± 2.76和54.45 ± 3.39 nmol / L）。与反应性和非常反应性组（分别为14.4 ± 0.2、13.6 ± 0.4和13.2 ± 0.2 mm）相比，平静组在TAI当天具有最大的卵泡直径。与第30天（分别为56.4％，57.0％，51.0％）和第60天（分别为50.0％，47.3％，42.7％）的高反应性组相比，平静和反应性组的P / TAI更高。总之，气质影响提交给TAI协议的牛雌性Bos taurus indicus的繁殖效率。