Background The formation of a nanotube layer on a titanium nanotube (N-Ti) plate facilitates an active reaction between bone cells and the material surface via efficient delivery of the surface materials of the dental implant into the tissues. Studies have reported that Korean Red Ginseng extracts (KRGEs) are involved in a variety of pharmacological activities: we investigated whether implantation with a KRGE-loaded N-Ti miniimplant affects osteogenesis and osseointegration. Methods KRGE-loaded nanotubes were constructed by fabrication on pure Ti via anodization, and MC3T3-E1 cells were cultured on the N-Ti. N-Ti implants were subsequently placed on a rat's edentulous mandibular site. New bone formation and bone mineral density were measured to analyze osteogenesis and osseointegration. Results KRGE-loaded N-Ti significantly increased the proliferation and differentiation of MC3T3-E1 cells compared with cells on pure Ti without any KRGE loading. After 1–4 weeks, the periimplant tissue in the edentulous mandibular of the healed rat showed a remarkable increase in new bone formation and bone mineral density. In addition, high levels of the bone morphogenesis protein-2 and bone morphogenesis protein-7, besides collagen, were expressed in the periimplant tissues. Conclusion Our findings suggest that KRGE-induced osteogenesis and osseointegration around the miniimplant may facilitate the clinical application of dental implants.

中文翻译：

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韩国红参提取物在种植牙中的骨重塑作用

背景技术在钛纳米管（N-Ti）板上形成纳米管层通过将牙种植体的表面材料有效递送到组织中来促进骨细胞和材料表面之间的积极反应。研究报告称，韩国红参提取物 (KRGE) 参与多种药理活性：我们研究了植入 KRGE 负载的 N-Ti 微型植入物是否会影响成骨和骨整合。方法在纯Ti上阳​​极氧化制备KRGE负载纳米管，在N-Ti上培养MC3T3-E1细胞。随后将 N-Ti 植入物放置在大鼠无牙颌下颌部位。测量新骨形成和骨矿物质密度以分析成骨和骨整合。结果与没有任何 KRGE 负载的纯 Ti 细胞相比，负载 KRGE 的 N-Ti 显着增加了 MC3T3-E1 细胞的增殖和分化。1-4周后，愈合大鼠下颌缺牙区的种植体周围组织显示出新骨形成和骨矿物质密度显着增加。此外，除了胶原蛋白外，在种植体周围组织中还表达了高水平的骨形态发生蛋白 2 和骨形态发生蛋白 7。结论 我们的研究结果表明，KRGE 诱导的小种植体周围的成骨和骨整合可能有助于牙种植体的临床应用。愈合大鼠下颌无牙颌的种植体周围组织显示新骨形成和骨矿物质密度显着增加。此外，除了胶原蛋白外，在种植体周围组织中还表达了高水平的骨形态发生蛋白 2 和骨形态发生蛋白 7。结论 我们的研究结果表明，KRGE 诱导的小种植体周围的成骨和骨整合可能有助于牙种植体的临床应用。愈合大鼠下颌无牙颌的种植体周围组织显示新骨形成和骨矿物质密度显着增加。此外，除了胶原蛋白外，在种植体周围组织中还表达了高水平的骨形态发生蛋白 2 和骨形态发生蛋白 7。结论 我们的研究结果表明，KRGE 诱导的小种植体周围的成骨和骨整合可能有助于牙种植体的临床应用。