Abstract Cereals and pulses often contribute to the intake of Fermentable Oligo-, Di-, Monosaccharides, and Polyols (FODMAPs) due to high amounts of fructans or galactooligosaccharides (GOS). FODMAPs can trigger symptoms of Irritable Bowel Syndrome (IBS) and therefore, the development of foods and beverages with a lower FODMAP-content are favourable for IBS patients. Enzyme technology is a promising tool to reduce the FODMAP-content in foods and to maintain product quality. This fundamental study investigates the efficiency of invertase, inulinase, and α-galactosidase as potential food additives to reduce the total FODMAP content of food ingredients. Extracts of high FODMAP ingredients, such as wheat and lentil, and standard solutions of various fructans and GOS were incubated with invertase, inulinase and α-galactosidase for 1 h and 2 h. Contents of oligosaccharides before and after treatment and related IBS-triggering reaction products were quantified using ion chromatography. Inulinase showed a high degradation yield (over 90% of degradation) for both GOS and fructans. For invertase only low degradation yields were measured. α-Galactosidase showed the highest efficiency in decomposing GOS (100% of degradation) and led to non-IBS triggering degradation products. This indicates a high potential for a combined inulinase/α-galactosidase treatment for products containing both fructans and GOS.

中文翻译：

---

通过应用β-呋喃果糖苷酶和α-半乳糖苷酶酶促降解FODMAPS-基础研究

摘要 由于大量果聚糖或低聚半乳糖 (GOS)，谷物和豆类通常有助于摄入可发酵的低聚糖、二糖、单糖和多元醇 (FODMAP)。FODMAP 可引发肠易激综合征 (IBS) 的症状，因此，开发低 FODMAP 含量的食品和饮料有利于 IBS 患者。酶技术是降低食品中 FODMAP 含量和保持产品质量的有前途的工具。这项基础研究调查了转化酶、菊粉酶和 α-半乳糖苷酶作为潜在食品添加剂以降低食品成分中 FODMAP 总含量的效率。将小麦和扁豆等高 FODMAP 成分的提取物以及各种果聚糖和 GOS 的标准溶液与转化酶、菊粉酶和 α-半乳糖苷酶孵育 1 小时和 2 小时。使用离子色谱法对处理前后的低聚糖含量和相关的 IBS 触发反应产物进行定量。菊粉酶对 GOS 和果聚糖均显示出高降解率（超过 90% 的降解）。对于转化酶，仅测量到低降解产率。α-半乳糖苷酶在分解 GOS（100% 降解）方面表现出最高效率，并导致非 IBS 触发降解产物。这表明组合菊粉酶/α-半乳糖苷酶处理含有果聚糖和 GOS 的产品的潜力很大。α-半乳糖苷酶在分解 GOS（100% 降解）方面表现出最高效率，并导致非 IBS 触发降解产物。这表明组合菊粉酶/α-半乳糖苷酶处理含有果聚糖和 GOS 的产品的潜力很大。α-半乳糖苷酶在分解 GOS（100% 降解）方面表现出最高效率，并导致非 IBS 触发降解产物。这表明组合菊粉酶/α-半乳糖苷酶处理含有果聚糖和 GOS 的产品的潜力很大。