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Characterization of centromeric satellite DNAs (MALREP) in the Asian swamp eel (Monopterus albus) suggests the possible origin of repeats from transposable elements.
Genomics ( IF 4.4 ) Pub Date : 2020-05-27 , DOI: 10.1016/j.ygeno.2020.05.024
Aorarat Suntronpong 1 , Worapong Singchat 1 , Worarat Kruasuwan 2 , Ornjira Prakhongcheep 2 , Siwapech Sillapaprayoon 1 , Narongrit Muangmai 3 , Suthasinee Somyong 4 , Chantra Indananda 2 , Ekaphan Kraichak 5 , Surin Peyachoknagul 2 , Kornsorn Srikulnath 6
Affiliation  

Centromeric satellite DNA (cen-satDNA) sequences of the Asian swamp eel (Monopterus albus) were characterized. Three GC-rich cen-satDNA sequences were detected as a 233 bp MALREP-A and a 293 bp MALREP-B localized to all chromosomes, and a 293 bp MALREP-C distributed on eight chromosome pairs. Sequence lengths of MALREP-B and MALREP-C were 60 bp larger than that of MALREP-A, showing partial homology with core sequences (233 bp). Size differences between MALREP-A and MALREP-B/C suggest the possible occurrence of two satDNA families. The presence of an additional 60 bp in MALREP-B/C resulted from an ancient dimer of 233 bp monomers and subsequent mutation and homogenization between the two monomers. All MALREPs showed partial homology with transposable elements (TEs), suggesting that the MALREPs originated from the TEs. The MALREPs might have been acquired in the Asian swamp eel, thereby promoting fixation in the species.



中文翻译:

亚洲沼泽鳗 (Monopterus albus) 中着丝粒卫星 DNA (MALREP) 的特征表明可能来自转座元件的重复序列。

亚洲沼泽鳗 ( Monopterus albus ) 的着丝粒卫星 DNA (cen-satDNA) 序列) 进行了表征。检测到三个富含 GC 的 cen-satDNA 序列为 233 bp MALREP-A 和 293 bp MALREP-B,位于所有染色体上,293 bp MALREP-C 分布在八对染色体上。MALREP-B 和 MALREP-C 的序列长度比 MALREP-A 大 60 bp,显示与核心序列(233 bp)部分同源。MALREP-A 和 MALREP-B/C 之间的大小差异表明可能存在两个 satDNA 家族。MALREP-B/C 中额外 60 bp 的存在是由 233 bp 单体的古老二聚体和随后的两个单体之间的突变和同质化引起的。所有 MALREPs 与转座因子 (TEs) 显示部分同源性,表明 MALREPs 起源于 TEs。MALREPs 可能是在亚洲沼泽鳗鱼中获得的,从而促进了对该物种的固定。

更新日期:2020-05-27
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