Buffalo is an amazing ruminant with high tolerance to low-quality roughage. Due to the special breeding environment, there are few reports on the digestibility of roughage in buffalo because it is difficult to quantify ingestion and egestion. To find more applicable method to determine the digestibility of low-quality roughage in buffalo, this study was conducted to compare total feces collection (TFC) method with the following three indirect techniques: Cr₂O₃ (chromic oxide), AIA (acid-insoluble ash), and ADL (acid detergent lignin), to determine rice straw digestibility in buffalo. Six non-pregnant, non-lactating female buffaloes were used in this experiment and the nutritional compositions of the rice straw and feces were measured. Using Cr₂O₃ and AIA methods, the digestibility of dry matter (DM), ash, organic matter (OM), nitrogen free extract (NFE), crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (CEL), hemicellulose (HC), and ADL did not have statistically significant differences compared with TFC (P > 0.05). However, the digestibility of DM, ash, OM, NFE, EE, CF, NDF, ADF, and ADL determined using ADL method were significantly lower than those using the TFC (P < 0.05). The feces recovery of Cr₂O₃ and AIA was 95.89% and 97.14%, which were higher than that of ADL (88.90%). In summary, compare with ADL method, TFC, Cr₂O₃, and AIA methods are more accurate to determine the roughage digestibility of buffalo. Furthermore, Cr₂O₃ and AIA methods are applicable and convenient to evaluate the roughage digestibility of buffalo under extensive feeding system.

水牛城是一种出色的反刍动物，对低质量的粗饲料具有很高的耐受性。由于特殊的繁殖环境，关于水牛粗饲料消化率的报道很少，因为难以量化其摄食和放牧。为了找到更适合的方法来确定水牛中劣质粗饲料的消化率，本研究将总粪便收集（TFC）方法与以下三种间接技术进行了比较：Cr ₂ O ₃（氧化铬），AIA（酸不溶性灰分）和ADL（酸性洗涤剂木质素）来确定稻草在水牛中的消化率。在该实验中使用了六个不怀孕，不哺乳的雌性水牛，并测量了稻草和粪便的营养成分。使用Cr ₂ O ₃和AIA方法，干物质（DM），灰分，有机物（OM），无氮提取物（NFE），粗蛋白（CP），醚提取物（EE），粗纤维（CF），中性洗涤剂纤维（与TFC相比，NDF），酸性洗涤剂纤维（ADF），纤维素（CEL），半纤维素（HC）和ADL的差异无统计学意义（P  > 0.05）。但是，使用ADL方法测定的DM，灰分，OM，NFE，EE，CF，NDF，ADF和ADL的消化率显着低于使用TFC的消化率（P  <0.05）。粪便中Cr ₂ O ₃和AIA的回收率分别为95.89％和97.14％，高于ADL的88.90％。总之，与ADL方法，TFC，Cr ₂ O _{3进行比较}和AIA方法可以更准确地确定水牛的粗消化率。此外，Cr ₂ O ₃和AIA方法适用于在广泛饲喂系统下评估水牛的粗消化率，并且方便。